Matches in SemOpenAlex for { <https://semopenalex.org/work/W2258542015> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2258542015 endingPage "9" @default.
- W2258542015 startingPage "1482" @default.
- W2258542015 abstract "We investigated serotonin stimulated palmitoylation of G alpha subunits in rat brain cerebrocortical membranes. Serotonin dose dependently stimulated palmitoylation of membrane G alpha proteins. The highest [3H] palmitate incorporation observed was by G alpha-q (7-fold), followed by G alpha-o (5-fold), G alpha-i (4-fold) and G alpha-s (3-fold) and these increases in palmitoylation were blocked by methiothipin, a serotonin receptor antagonist. Isoproterenol selectively stimulated G alpha-s palmitoylation which was blocked by propranalol. Immunoprecipitates of palmitoylated G alpha subunits yielded single labeled bands on SDS-PAGE. In an attempt to define the sequence of palmitoylation/depalmitoylation that follows receptor stimulation, nonreceptor mediated palmitoylation was carried out in the presence of guanine nucleotides and receptor mediated G alpha depalmitoylation was then monitored. Receptor stimulation did not result in depalmitoylation when membranes were prelabeled with [3H] palmitic acid in the presence of the nonhydrolyzable analogue of GTP, Gpp(NH)p. However, serotonin receptor stimulation in the presence of guanine nucleotides, depalmitoylated (90%) membrane G alpha proteins when prelabeled in the presence of GTP. Coimmunoprecipitation experiments revealed decrease in G beta immunoreactivity associated with G alpha immunoprecipitates obtained from membranes prelabeled in presence of GTP prior to reincubation with Gpp(NH)p and serotonin. These observations suggest that receptor occupation results in depalmitoylation of the trimer, followed by guanine nucleotide exchange and dissociation of the alpha subunit from beta-gamma dimer and that the activated alpha subunit is a substrate for repalmitoylation." @default.
- W2258542015 created "2016-06-24" @default.
- W2258542015 creator A5029990707 @default.
- W2258542015 creator A5031247002 @default.
- W2258542015 creator A5058595243 @default.
- W2258542015 date "1998-09-01" @default.
- W2258542015 modified "2023-09-23" @default.
- W2258542015 title "Serotonin-mediated palmitoylation and depalmitoylation of G alpha proteins in rat brain cortical membranes." @default.
- W2258542015 cites W1499411585 @default.
- W2258542015 cites W1523056580 @default.
- W2258542015 cites W1540505454 @default.
- W2258542015 cites W1546743612 @default.
- W2258542015 cites W1588431496 @default.
- W2258542015 cites W1623804763 @default.
- W2258542015 cites W1775749144 @default.
- W2258542015 cites W1970196085 @default.
- W2258542015 cites W1970716791 @default.
- W2258542015 cites W1972760976 @default.
- W2258542015 cites W1977598234 @default.
- W2258542015 cites W1978686382 @default.
- W2258542015 cites W1984212762 @default.
- W2258542015 cites W2023207364 @default.
- W2258542015 cites W2036804347 @default.
- W2258542015 cites W2036842366 @default.
- W2258542015 cites W2037085464 @default.
- W2258542015 cites W2051750791 @default.
- W2258542015 cites W2055748995 @default.
- W2258542015 cites W2057779233 @default.
- W2258542015 cites W2100837269 @default.
- W2258542015 cites W2101108802 @default.
- W2258542015 cites W2128806251 @default.
- W2258542015 cites W2155380978 @default.
- W2258542015 cites W2279628140 @default.
- W2258542015 cites W2310682394 @default.
- W2258542015 cites W2409197986 @default.
- W2258542015 cites W2462244652 @default.
- W2258542015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9732414" @default.
- W2258542015 hasPublicationYear "1998" @default.
- W2258542015 type Work @default.
- W2258542015 sameAs 2258542015 @default.
- W2258542015 citedByCount "6" @default.
- W2258542015 countsByYear W22585420152013 @default.
- W2258542015 countsByYear W22585420152018 @default.
- W2258542015 crossrefType "journal-article" @default.
- W2258542015 hasAuthorship W2258542015A5029990707 @default.
- W2258542015 hasAuthorship W2258542015A5031247002 @default.
- W2258542015 hasAuthorship W2258542015A5058595243 @default.
- W2258542015 hasConcept C104292427 @default.
- W2258542015 hasConcept C104317684 @default.
- W2258542015 hasConcept C118716 @default.
- W2258542015 hasConcept C146727910 @default.
- W2258542015 hasConcept C159110408 @default.
- W2258542015 hasConcept C170493617 @default.
- W2258542015 hasConcept C181199279 @default.
- W2258542015 hasConcept C2775944032 @default.
- W2258542015 hasConcept C2779201268 @default.
- W2258542015 hasConcept C49453240 @default.
- W2258542015 hasConcept C55493867 @default.
- W2258542015 hasConcept C64943373 @default.
- W2258542015 hasConcept C71924100 @default.
- W2258542015 hasConcept C80631254 @default.
- W2258542015 hasConcept C83730767 @default.
- W2258542015 hasConcept C86803240 @default.
- W2258542015 hasConceptScore W2258542015C104292427 @default.
- W2258542015 hasConceptScore W2258542015C104317684 @default.
- W2258542015 hasConceptScore W2258542015C118716 @default.
- W2258542015 hasConceptScore W2258542015C146727910 @default.
- W2258542015 hasConceptScore W2258542015C159110408 @default.
- W2258542015 hasConceptScore W2258542015C170493617 @default.
- W2258542015 hasConceptScore W2258542015C181199279 @default.
- W2258542015 hasConceptScore W2258542015C2775944032 @default.
- W2258542015 hasConceptScore W2258542015C2779201268 @default.
- W2258542015 hasConceptScore W2258542015C49453240 @default.
- W2258542015 hasConceptScore W2258542015C55493867 @default.
- W2258542015 hasConceptScore W2258542015C64943373 @default.
- W2258542015 hasConceptScore W2258542015C71924100 @default.
- W2258542015 hasConceptScore W2258542015C80631254 @default.
- W2258542015 hasConceptScore W2258542015C83730767 @default.
- W2258542015 hasConceptScore W2258542015C86803240 @default.
- W2258542015 hasIssue "3" @default.
- W2258542015 hasLocation W22585420151 @default.
- W2258542015 hasOpenAccess W2258542015 @default.
- W2258542015 hasPrimaryLocation W22585420151 @default.
- W2258542015 hasRelatedWork W1504083190 @default.
- W2258542015 hasRelatedWork W1536363529 @default.
- W2258542015 hasRelatedWork W1540505454 @default.
- W2258542015 hasRelatedWork W1960199343 @default.
- W2258542015 hasRelatedWork W1999151196 @default.
- W2258542015 hasRelatedWork W2005690028 @default.
- W2258542015 hasRelatedWork W2061853525 @default.
- W2258542015 hasRelatedWork W2083617532 @default.
- W2258542015 hasRelatedWork W2172203448 @default.
- W2258542015 hasRelatedWork W2258542015 @default.
- W2258542015 hasVolume "286" @default.
- W2258542015 isParatext "false" @default.
- W2258542015 isRetracted "false" @default.
- W2258542015 magId "2258542015" @default.
- W2258542015 workType "article" @default.