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- W2258581570 abstract "Lycopene can be synthesized from FPP in E. coli by introduction of crtE, crtB, and crtI.β-Carotene, zeaxanthin, and astaxanthin can be produced from the lycopene-producingE. coli by introduction of crtY, crtW, and crtZ. The plasmids, pT-LYCm4 for lycopene,pT-HB for β-carotene, and pT-ZUM6 for zeaxanthin were constructed to produce eachcarotenoid in E. coli. β-Carotene can be converted to astaxanthin via zeaxanthin by crtZ,or via canthaxanthin by crtW. For production of astaxanthin in E. coli (pT-ZUM6), crtWand crtZ genes were cloned from 6 bacteria, Brevundimonas aurantiaca,Brevundimonas vesicularis, Novosphingobium aromaticivorum, Parvularculaburmudensis, Robiginitalea biformata, and Xanthobacter autotrophicus. Astaxanthin of5.4 mg/L and other intermediate carotenoids were produced from the E. coli (pT-ZUM6)harboring crtW of B. vesicularis. There are various intermediate carotenoids on thebiosynthetic pathway from β-carotene to astaxanthin. No significant activities werenotobserved from the crtZ and crtW of the other bacteria. This work was supported by the21C Frontier Microbial Genomics and Applications Center Program, EB-NCRC (GrantNo. R15-2003-012-02001-0), Brain Pool program and BK21 program of Korea." @default.
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- W2258581570 date "2008-10-01" @default.
- W2258581570 modified "2023-09-24" @default.
- W2258581570 title "Effect of crtW and crtZ on astaxanthin production from Escherichia coli harboring β-carotene pathway" @default.
- W2258581570 hasPublicationYear "2008" @default.
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