FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2258678574> ?p ?o ?g. }

• W2258678574 endingPage "736" @default.
• W2258678574 startingPage "727" @default.
• W2258678574 abstract "To investigate substrates for cyclic AMP-dependent protein kinase in intact islets of Langerhans, batches of islets were incubated with [32P]Pi for 1 h in the presence of 10 mM-glucose; the adenylate cyclase activator forskolin, which in parallel experiments was shown to increase islet cyclic AMP content and insulin release, was then added. Islets were homogenized and subcellular fractions prepared by differential centrifugation. Phosphopeptides were electrophoresed on sodium dodecyl sulphate/polyacrylamide gels and quantified by autoradiography and densitometry. Within 5 min forskolin caused increased labelling of Mr-25 000 and -30 000 cytosolic and Mr-23 000 and -32 000 particulate peptides; a rapid decrease in phosphorylation of Mr-18 000 and -34 000 cytosolic peptides was also observed. In addition, rather slower phosphorylation occurred of the Mr-15 000 peptide previously identified as histone H3 [Christie & Ashcroft (1984) Biochem. J. 218, 87-99]. When similar subcellular fractions were incubated with [gamma-32P]ATP and purified catalytic subunit of cyclic AMP-dependent protein kinase, peptides phosphorylated included cytosolic species of Mr 25 000 and 30 000 and particulate species of Mr 23 000 and 32 000. The distribution of RNA in the subcellular fractions suggested that the Mr-32 000 species could be a ribosomal protein. The 24 000 g pellet was heterogeneous, as judged by marker assays, and was therefore fractionated further by Percoll-density-gradient centrifugation. The peak containing the Mr-23 000 peptide was resolved from marker enzymes for plasma membranes, mitochondria and endoplasmic reticulum and coincided with a peak for insulin: hence the Mr-23 000 peptide is likely to be a secretory-granule component. The study demonstrates that the potentiation of insulin release that occurs when islet cyclic AMP is increased is accompanied by rapid phosphorylation of specific islet substrates for cyclic AMP-dependent protein kinase. The data are consistent with the hypothesis that protein phosphorylation is involved in the regulation of insulin secretion." @default.
• W2258678574 created "2016-06-24" @default.
• W2258678574 creator A5020914139 @default.
• W2258678574 creator A5045884090 @default.
• W2258678574 date "1985-05-01" @default.
• W2258678574 modified "2023-09-25" @default.
• W2258678574 title "Substrates for cyclic AMP-dependent protein kinase in islets of Langerhans. Studies with forskolin and catalytic subunit" @default.
• W2258678574 cites W123088864 @default.
• W2258678574 cites W1548285607 @default.
• W2258678574 cites W1573741209 @default.
• W2258678574 cites W1582080277 @default.
• W2258678574 cites W1589882347 @default.
• W2258678574 cites W1596707465 @default.
• W2258678574 cites W1865773512 @default.
• W2258678574 cites W1881721492 @default.
• W2258678574 cites W1967154786 @default.
• W2258678574 cites W1995201350 @default.
• W2258678574 cites W2003340009 @default.
• W2258678574 cites W2015776507 @default.
• W2258678574 cites W2016501481 @default.
• W2258678574 cites W2018289835 @default.
• W2258678574 cites W2018776302 @default.
• W2258678574 cites W2027466494 @default.
• W2258678574 cites W2043815788 @default.
• W2258678574 cites W2046203117 @default.
• W2258678574 cites W2046284631 @default.
• W2258678574 cites W2048679997 @default.
• W2258678574 cites W2053583890 @default.
• W2258678574 cites W2057029840 @default.
• W2258678574 cites W2059716312 @default.
• W2258678574 cites W2074020540 @default.
• W2258678574 cites W2087318554 @default.
• W2258678574 cites W2100837269 @default.
• W2258678574 cites W2126230664 @default.
• W2258678574 cites W2128635872 @default.
• W2258678574 cites W2169477600 @default.
• W2258678574 cites W2212659187 @default.
• W2258678574 cites W2338200712 @default.
• W2258678574 cites W2398399841 @default.
• W2258678574 cites W2412604744 @default.
• W2258678574 cites W8404676 @default.
• W2258678574 cites W967130900 @default.
• W2258678574 doi "https://doi.org/10.1042/bj2270727" @default.
• W2258678574 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1144899" @default.
• W2258678574 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2988505" @default.
• W2258678574 hasPublicationYear "1985" @default.
• W2258678574 type Work @default.
• W2258678574 sameAs 2258678574 @default.
• W2258678574 citedByCount "28" @default.
• W2258678574 countsByYear W22586785742016 @default.
• W2258678574 crossrefType "journal-article" @default.
• W2258678574 hasAuthorship W2258678574A5020914139 @default.
• W2258678574 hasAuthorship W2258678574A5045884090 @default.
• W2258678574 hasBestOaLocation W22586785742 @default.
• W2258678574 hasConcept C104292427 @default.
• W2258678574 hasConcept C104317684 @default.
• W2258678574 hasConcept C11960822 @default.
• W2258678574 hasConcept C132946820 @default.
• W2258678574 hasConcept C153911025 @default.
• W2258678574 hasConcept C158617107 @default.
• W2258678574 hasConcept C170493617 @default.
• W2258678574 hasConcept C180526460 @default.
• W2258678574 hasConcept C181199279 @default.
• W2258678574 hasConcept C27239569 @default.
• W2258678574 hasConcept C2778492296 @default.
• W2258678574 hasConcept C2779276759 @default.
• W2258678574 hasConcept C48679268 @default.
• W2258678574 hasConcept C55493867 @default.
• W2258678574 hasConcept C62478195 @default.
• W2258678574 hasConcept C7623868 @default.
• W2258678574 hasConcept C80631254 @default.
• W2258678574 hasConcept C86803240 @default.
• W2258678574 hasConcept C97029542 @default.
• W2258678574 hasConcept C98539663 @default.
• W2258678574 hasConceptScore W2258678574C104292427 @default.
• W2258678574 hasConceptScore W2258678574C104317684 @default.
• W2258678574 hasConceptScore W2258678574C11960822 @default.
• W2258678574 hasConceptScore W2258678574C132946820 @default.
• W2258678574 hasConceptScore W2258678574C153911025 @default.
• W2258678574 hasConceptScore W2258678574C158617107 @default.
• W2258678574 hasConceptScore W2258678574C170493617 @default.
• W2258678574 hasConceptScore W2258678574C180526460 @default.
• W2258678574 hasConceptScore W2258678574C181199279 @default.
• W2258678574 hasConceptScore W2258678574C27239569 @default.
• W2258678574 hasConceptScore W2258678574C2778492296 @default.
• W2258678574 hasConceptScore W2258678574C2779276759 @default.
• W2258678574 hasConceptScore W2258678574C48679268 @default.
• W2258678574 hasConceptScore W2258678574C55493867 @default.
• W2258678574 hasConceptScore W2258678574C62478195 @default.
• W2258678574 hasConceptScore W2258678574C7623868 @default.
• W2258678574 hasConceptScore W2258678574C80631254 @default.
• W2258678574 hasConceptScore W2258678574C86803240 @default.
• W2258678574 hasConceptScore W2258678574C97029542 @default.
• W2258678574 hasConceptScore W2258678574C98539663 @default.
• W2258678574 hasIssue "3" @default.
• W2258678574 hasLocation W22586785741 @default.
• W2258678574 hasLocation W22586785742 @default.
• W2258678574 hasLocation W22586785743 @default.

• next