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• W2258704056 abstract "Double-stranded RNA (dsRNA) viruses possess a segmented dsRNA genome and a number of RNA-dependent RNA polymerases (RdRps) enclosed in a capsid. Until now, the precise structures of genomes and RdRps within the capsids have been unknown. Here we report the structures of RdRps and associated RNAs within nontranscribing and transcribing cypoviruses (NCPV and TCPV, respectively), using a combination of cryo-electron microscopy (cryo-EM) and a symmetry-mismatch reconstruction method. The RdRps and associated RNAs appear to exhibit a pseudo-D3 symmetric organization in both NCPV and TCPV. However, the molecular interactions between RdRps and the genomic RNA were found to differ in these states. Our work provides insight into the mechanisms of the replication and transcription in dsRNA viruses and paves a way for structural determination of lower-symmetry complexes enclosed in higher-symmetry structures." @default.
• W2258704056 created "2016-06-24" @default.
• W2258704056 creator A5044997357 @default.
• W2258704056 creator A5055861040 @default.
• W2258704056 date "2015-09-18" @default.
• W2258704056 modified "2023-10-14" @default.
• W2258704056 title "Cryo-EM shows the polymerase structures and a nonspooled genome within a dsRNA virus" @default.
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• W2258704056 doi "https://doi.org/10.1126/science.aaa4938" @default.
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