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- W2258863669 abstract "目的：观察针对丙型肝炎病毒（HCV）5’非编码区（NCR）内源性核糖体进入位点（IRES）的锁核酸核酶对HepG2.9706细胞中HCVRNA表达的抑制作用. 方法：设计合成针对HCV5＇-NCR区IRES位点的DNAzyme、硫代修饰DNAzyme及LNAzyme.实验设对照组与实验组.对照组包括空白对照组、脂质体对照组、无关DNAzyme对照组、裸DNAzyme对照组.实验组包括脂质体-DNAzyme组、脂质体-硫代修饰DNAzyme组、脂质体-LNAzyme组.观察用药后1、3、5、7d时,对HepG2.9706细胞的HCVRNA及荧光素酶基因表达的抑制效应. 结果：脂质体包裹的DNAzyme、硫代修饰DNAzyme及LNAzyme对HCVRNA表达均有抑制作用,平均抑制率分别为28.10%、35.25%和44.58%.对荧光素酶基因表达也有抑制作用,平均抑制率分别为31.18%、40.69%和52.33%.与对照组比较均有显著性差异（P〈0.05）.对HepG2.9706细胞未见明显细胞毒性作用. 结论：LNAzyme对HepG2.9706细胞内HCVRNA的表达具有显著抑制作用,且优于硫代修饰的DNAzyme,是一类特异的高效抗HCV分子药物." @default.
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- W2258863669 date "2010-01-01" @default.
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- W2258863669 title "LNAzyme targeting the HCV internal ribosome entry site inhibits HCV RNA expression in HepG2.9706 cells" @default.
- W2258863669 doi "https://doi.org/10.11569/wcjd.v18.i20.2132" @default.
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