FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2258928506> ?p ?o ?g. }

• W2258928506 abstract "Overexpression of Arabidopsis thaliana purple acid phosphatases AtPAP2 in Arabidopsis can promote plant growth. The overexpression (OE) lines flower earlier, grow faster, and contain more ATP, sucrose and glucose. The seed yields and silique numbers of OE lines are also more than the control lines (Sun et al., 2012a). In this study, we compared the leaf transcriptomes of 20-d-old transgenic and wild-type Arabidopsis grown under long day (16h/8h) condition. Total RNAs were collected at three time points: end of night (t=0 hr), one hour after light was turned on (t=1 hr), and eight hours after light was turned on (t=8 hr). AtPAP2 is dually targeted to chloroplasts and mitochondria. To study the RNA encoded by chloroplast and mitochondrial genomes, ribosomal RNAs were removed before sequencing. Approximate 65 million clean reads (~6Gbp) were obtained by Illumina HiSeqTM 2000 sequencing from each library. In total, after assembly 29,435 transcripts are detected from the six libraries. More genes are suppressed in OE leaves (vs. WT) at all the three time points, 1,623 (downregulated) versus 945 (up-regulated), 1,908 (down-regulated) versus 712 (up-regulated) and 1,642 (down-regulated) versus 824 (up-regulated) at t=0, 1 and 8 hours, respectively. Expression profiles of light-induced transcripts based on K-means clustering were analyzed. There are significant changes in the transcription levels of various components of photosystems, light-harvesting chlorophyll protein complexes (LHC), respiratory complexes, RNA polymerases and ribosomes. Our data provide systemic portraits of global changes in Arabidopsis transcriptome exerted by dark to light transition (external energy input) and high internal energy status. Comparative transcriptome analysis of energy-rich Arabidopsis thaliana under dark and light conditions Chao LIANG and Boon Leong LIM* vechao@hku.hk School of Biological Sciences, the University of Hong Kong, Hong Kong, China Fig. 1 Growth phenotypes of four-wk-old Arabidopsis under long day (16hr light/8hr dark)) conditions. WT, wild type; T-DNA, AtPAP2 mutant line; OE, AtPAP2 over-expression lines (Sun et al., 2012b). Arabidopsis thaliana ecotypes Columbia (Col-0) (wild type: WT), AtPAP2 overexpressors (OE) in Col-0 background were used in this study (Sun et al. 2012b) . Arabidopsis seeds of WT and OE7 lines were surface sterilized and plated on Murashige and Skoog medium supplemented with 2% (w/v) sucrose for 10 days before the seedling were transferred to soil under 16 hours light (22°C) / 8 hours dark (18°C) period in a growth chamber at a light intensity of 120–150 μmol m-2 s-1. Leaves of 20-day-old plants without bolting were immediately frozen in liquid nitrogen for RNA extraction. Leaves were harvested at three different time points: t=0 hr (end of night), t=1 hr (one hour after light turn on) and t=8 hr (eight hours after light turn on) respectively. Flowchart Total RNA Removal of Ribosome RNA Raw Data Allignment with reference gene and genome file (SOAP aligner/SOAP2) Clean Reads QC Expression level(RPKM) Cluster analysisc Gene ontology analysisc KEGG pathway analysis Background Methodology" @default.
• W2258928506 created "2016-06-24" @default.
• W2258928506 creator A5048046659 @default.
• W2258928506 creator A5052671919 @default.
• W2258928506 date "2013-08-08" @default.
• W2258928506 modified "2023-10-04" @default.
• W2258928506 title "Comparative transcriptome analysis of energy-rich Arabidopsis thaliana under dark and light conditions" @default.
• W2258928506 doi "https://doi.org/10.7490/f1000research.1093942.1" @default.
• W2258928506 hasPublicationYear "2013" @default.
• W2258928506 type Work @default.
• W2258928506 sameAs 2258928506 @default.
• W2258928506 citedByCount "0" @default.
• W2258928506 crossrefType "journal-article" @default.
• W2258928506 hasAuthorship W2258928506A5048046659 @default.
• W2258928506 hasAuthorship W2258928506A5052671919 @default.
• W2258928506 hasConcept C104317684 @default.
• W2258928506 hasConcept C120858795 @default.
• W2258928506 hasConcept C143065580 @default.
• W2258928506 hasConcept C150194340 @default.
• W2258928506 hasConcept C162317418 @default.
• W2258928506 hasConcept C2775988993 @default.
• W2258928506 hasConcept C2779491563 @default.
• W2258928506 hasConcept C54355233 @default.
• W2258928506 hasConcept C69305403 @default.
• W2258928506 hasConcept C86803240 @default.
• W2258928506 hasConceptScore W2258928506C104317684 @default.
• W2258928506 hasConceptScore W2258928506C120858795 @default.
• W2258928506 hasConceptScore W2258928506C143065580 @default.
• W2258928506 hasConceptScore W2258928506C150194340 @default.
• W2258928506 hasConceptScore W2258928506C162317418 @default.
• W2258928506 hasConceptScore W2258928506C2775988993 @default.
• W2258928506 hasConceptScore W2258928506C2779491563 @default.
• W2258928506 hasConceptScore W2258928506C54355233 @default.
• W2258928506 hasConceptScore W2258928506C69305403 @default.
• W2258928506 hasConceptScore W2258928506C86803240 @default.
• W2258928506 hasLocation W22589285061 @default.
• W2258928506 hasOpenAccess W2258928506 @default.
• W2258928506 hasPrimaryLocation W22589285061 @default.
• W2258928506 hasRelatedWork W1524154007 @default.
• W2258928506 hasRelatedWork W1964695047 @default.
• W2258928506 hasRelatedWork W1967479392 @default.
• W2258928506 hasRelatedWork W1976799943 @default.
• W2258928506 hasRelatedWork W2005143168 @default.
• W2258928506 hasRelatedWork W2168728371 @default.
• W2258928506 hasRelatedWork W2272342866 @default.
• W2258928506 hasRelatedWork W2349633003 @default.
• W2258928506 hasRelatedWork W2407049343 @default.
• W2258928506 hasRelatedWork W2507161241 @default.
• W2258928506 hasRelatedWork W2802042274 @default.
• W2258928506 hasRelatedWork W3000403684 @default.
• W2258928506 hasRelatedWork W3102359546 @default.
• W2258928506 hasVolume "4" @default.
• W2258928506 isParatext "false" @default.
• W2258928506 isRetracted "false" @default.
• W2258928506 magId "2258928506" @default.
• W2258928506 workType "article" @default.