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- W2258997430 abstract "High quality genomic DNA of a moderately halophilic bacterial strain Halomonas sp. BYS-1 was extracted. The promoter library of BYS-1 was constructed in E. coli DH5α with pUC-mpd as the promoter probe vector by shotgun-cloning method. 17 positive clones designated as P1~P17 were obtained by screening. Their methyl parathion hydrolase (MPH) activity was assayed to compare the promoting strength of the promoters.The results showed that the promoter of mpd in P3 was the strongest with MPH activity of 2 554.3 U/mg,while the promoter of mpd in P17 was the weakest of 68.3 U/mg. The inserting fragments in the recombinant plasmids from P3, P8 and P17 were sequenced and subjected to online promoter prediction.Fig 4, Tab 1, Ref 17" @default.
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- W2258997430 date "2005-12-25" @default.
- W2258997430 modified "2023-09-24" @default.
- W2258997430 title "中度嗜盐菌Halomonas sp．BYS-1启动子的克隆和测序" @default.
- W2258997430 hasPublicationYear "2005" @default.
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