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- W2259569314 abstract "Abstract A plasmid containing promoter-deleted inactive β-galactosidase gene [1] was used to select promoters of the pEP121 plasmid [2]. Colonies of cells harboring reactivated β-galactosidase gene were identified by their red color on McConkey plates. The quantitative amounts of β-galactosidase produced in each clone were estimated by assaying enzyme activity and by measuring the specific β-galactosidase protein following fractionation of total cells′ proteins on polyacrylamide gel. A wide range of enzyme activities was observed. The most active promoter isolated was shown to promote β-galactosidase production more efficiently, compared with the original β-galactosidase promoter, amounting to 20% of all cell proteins. Such highly active promoters may be utilized in the future, to promote expression of cloned genes in bacteria." @default.
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- W2259569314 date "1982-06-01" @default.
- W2259569314 modified "2023-10-15" @default.
- W2259569314 title "Screening for Highly Active Plasmid Promoters via Fusion to β-Galactosidase Gene" @default.
- W2259569314 doi "https://doi.org/10.1515/znc-1982-5-614" @default.
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