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- W226016404 abstract "A pair of primers with specificity to amplify PRL gene was designed and synthesized using conservative domain of mammalian PRL cDNA reported in GenBank.Total RNA was extracted from pituitary gland of Guangxi local buffaloes and the sequence of PRL cDNA was amplified and cloned using pMD 18-T vector.Through PCR and enzyme di-gestion,the sequence analysis of positive cloning indicated that the cDNA contained an ORF of 690 bp which codes 229 amino acids,of which the first 19 amino acids were signal peptide.By comparing with published prolactin gene sequence in GenBank with Blast function,the sequence showed highest identity with PRL gene,and the sequence homology of buffalo to cattle was found 98.4%.This proved that the cloned sequence was complete buffalo PRL gene sequence,and that has been submitted to GenBank(GenBank accession No.EF054878)." @default.
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- W226016404 date "2009-01-01" @default.
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- W226016404 title "Cloning and sequence analysis of the pituitary prolactin cDNA of buffalo." @default.
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