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- W2260759212 abstract "The current study was designed to examine calcium transport across the basolateral membrane of the enterocyte of control and diabetic rats. The slope of initial rate of ATP-dependent calcium uptake was significantly greater in controls than in diabetic rats (P < 0.001). Kinetic analysis of ATP-dependent calcium uptake showed a maximum velocity (Vmax) of 0.19 ± 0.02 and 0.37 ± 0.01 µmol · g protein−1 · s−1 in diabetic and control rats, respectively (P < 0.01). Km values were similar. Insulin therapy and calmodulin added to freeze-thawed basolateral membranes vesicles stimulated Vmax of ATP-dependent calcium uptake in diabetic membranes to values close to those of controls without a change in Km values. In contrast, the Ca++-Na+ exchange process was similar in both control and diabetic rats. These results suggest that the ATP-dependent calcium uptake process is decreased in diabetic animals. This decrease is stimulated by calmodulin or insulin therapy, implying a causal relationship between insulin deficiency and calmodulin activity in diabetes." @default.
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- W2260759212 date "1993-08-01" @default.
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- W2260759212 title "Calcium transport by basolateral membranes of diabetic rats" @default.
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- W2260759212 doi "https://doi.org/10.1093/ajcn/58.2.209" @default.
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