FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2260851941> ?p ?o ?g. }

• W2260851941 abstract "Cowpea is a major source of dietary protein in most African traditional diets, and the crop partially replenishes the soil nitrogen content as a result of its association with nitrogen-fixing Rhizobium spp, and is used as fodder. However, cowpea suffers from a wide range of production constraints such as viral diseases. Cowpea aphid-borne mosaic potyvirus (CABMV) is one of the most important viral pathogens of cowpea in major cowpea growing regions of the world and significantly reduces cowpea yields of resource poor farmers. In an effort to characterise a CABMV isolate from Zimbabwe, designated CABMV-Z3, using classical virology techniques the host range of CAMBV-Z3 was tested. The symptoms produced on susceptible Nicotiana bethamiana, N. clevelandii, Vigna unguiculata, Chenopodium amaranticolor and Ch. quinoa plants were described. Nicotiana glutinosa, N. tabacum, N. rustica, Phaseolus vulgaris and Cucumeris sativus were shown to be non-hosts of the virus. The CABMV-Z3 was purified for the first time and polyclonal antiserum was produced against the virus. Full-length cDNA clones of CABMV-Z3 were made by long-range reverse transcription-polymerase chain reaction (RT-PCR).Another aspect of the study was to determine the most effective virus resistance mechanism using the CABMV-Z3 coat protein (CP) gene, in tobacco as a model host since no reliable cowpea transformation protocol was available at the time. The 1.2 kb 3’ terminal region of CABMV-Z3 was amplified using RT-PCR technique and cloned into a PCR cloning vector pGEM-T, and named pGEM-CPRep. This clone was used as template in a PCR to amplify a CP gene optimised for expression in plants (CPk), an untranslatable CP gene with stop codons in all three reading frames (CPstop), an anti-sense CP (PC), and the central region of the CP gene (CPcore). All four amplification products were provided with promoters and terminators from expression cassette vector pCa2Nos, and ligated into the unique Hind III site of the binary plasmid pBI121, to result in plasmids pBI121-CPk, pBI121-CPstop, pBI121-PC and pBI121-CPcore. The constructs were used in Agrobacterium-mediated transformation of Nicotiana benthamiana leaf sections following the co-cultivation method. Regenerated plants were analyzed by PCR and Southern hybidization. R1 seedlings were assayed for kanamycin resistance and for presence of CP, and challenged with CAMBV-infected sap. Lines showing delayed symptom development, tolerance and recovery were identified but no line showing immunity was identified. This was significant resistance, since it affords protection to the plants during the crucial early stages of development and exerts little evolutionary pressure on the virus to evolve new strategies.The ultimate goal of the project was to produce cowpea lines that are resistant to CABMV. However, there was no efficient, reliable and reproducible cowpea transformation and regeneration system. A cowpea transformation protocol was developed and evaluated, utilizing the binary constructs specified above. The binary constructs were electrophoresed into the apical meristems of developing cowpea seedlings under various conditions including different voltage and current settings as well as pretreatment of seedlings with acid or plant growth regulators. Preliminary screening was done using GUS assays, PCR and Southern analysis, and the plants allowed to set seed. The T1 seeds were germinated in-vitro on MS plates. The results show that DNA delivery to meristematic cells was successful, leading to transgenic sectors of the plants. However, the chimeric nature of the plants poses a problem, as there is need for a reliable non-destructive mechanism to track the transgenic branches of the chimeras. This transformation method has the potential to avert some of the concerns often raised against transgenic plants since it does not necessarily require selectable marker and reporter genes, and avoids the expensive and laborious tissue culture step." @default.
• W2260851941 created "2016-06-24" @default.
• W2260851941 creator A5033435938 @default.
• W2260851941 date "2012-08-28" @default.
• W2260851941 modified "2023-09-23" @default.
• W2260851941 title "Characterisation of Cowpea aphid-borne mosaic virus (CABMV) and evaluation of pathogen derived resistance to the virus." @default.
• W2260851941 cites W105022716 @default.
• W2260851941 cites W106773467 @default.
• W2260851941 cites W1487077022 @default.
• W2260851941 cites W1491556959 @default.
• W2260851941 cites W1493879176 @default.
• W2260851941 cites W1508539657 @default.
• W2260851941 cites W1517642551 @default.
• W2260851941 cites W1545725760 @default.
• W2260851941 cites W1547843440 @default.
• W2260851941 cites W1552456601 @default.
• W2260851941 cites W1553868199 @default.
• W2260851941 cites W1594693961 @default.
• W2260851941 cites W1599213518 @default.
• W2260851941 cites W1607238965 @default.
• W2260851941 cites W1691287462 @default.
• W2260851941 cites W1792302550 @default.
• W2260851941 cites W1918169880 @default.
• W2260851941 cites W193914404 @default.
• W2260851941 cites W1953745208 @default.
• W2260851941 cites W1963788154 @default.
• W2260851941 cites W1965215839 @default.
• W2260851941 cites W1966931316 @default.
• W2260851941 cites W1968447599 @default.
• W2260851941 cites W1969494366 @default.
• W2260851941 cites W1969553476 @default.
• W2260851941 cites W1970983567 @default.
• W2260851941 cites W1971107036 @default.
• W2260851941 cites W1973204779 @default.
• W2260851941 cites W1974557838 @default.
• W2260851941 cites W1974666441 @default.
• W2260851941 cites W1974831611 @default.
• W2260851941 cites W1974860031 @default.
• W2260851941 cites W1975558119 @default.
• W2260851941 cites W1977091294 @default.
• W2260851941 cites W1977665102 @default.
• W2260851941 cites W1978223128 @default.
• W2260851941 cites W1978888783 @default.
• W2260851941 cites W1981632277 @default.
• W2260851941 cites W1983183098 @default.
• W2260851941 cites W1983420339 @default.
• W2260851941 cites W1984675152 @default.
• W2260851941 cites W1985661896 @default.
• W2260851941 cites W1986104107 @default.
• W2260851941 cites W1986136416 @default.
• W2260851941 cites W1986232581 @default.
• W2260851941 cites W1986587865 @default.
• W2260851941 cites W1987390135 @default.
• W2260851941 cites W1988460270 @default.
• W2260851941 cites W1989454102 @default.
• W2260851941 cites W1989753542 @default.
• W2260851941 cites W1992085874 @default.
• W2260851941 cites W1994545733 @default.
• W2260851941 cites W1994697216 @default.
• W2260851941 cites W1995273609 @default.
• W2260851941 cites W1998738791 @default.
• W2260851941 cites W1999889421 @default.
• W2260851941 cites W2001194640 @default.
• W2260851941 cites W2002779363 @default.
• W2260851941 cites W2003171497 @default.
• W2260851941 cites W2005030332 @default.
• W2260851941 cites W2006807779 @default.
• W2260851941 cites W2008206526 @default.
• W2260851941 cites W2008291539 @default.
• W2260851941 cites W2010285927 @default.
• W2260851941 cites W2010688536 @default.
• W2260851941 cites W2011791385 @default.
• W2260851941 cites W2011947972 @default.
• W2260851941 cites W2012209192 @default.
• W2260851941 cites W2013923998 @default.
• W2260851941 cites W2013931096 @default.
• W2260851941 cites W2015091566 @default.
• W2260851941 cites W2019471731 @default.
• W2260851941 cites W2020934256 @default.
• W2260851941 cites W2021947220 @default.
• W2260851941 cites W2024598619 @default.
• W2260851941 cites W2025300050 @default.
• W2260851941 cites W2025561511 @default.
• W2260851941 cites W2025738827 @default.
• W2260851941 cites W2026326687 @default.
• W2260851941 cites W2026437684 @default.
• W2260851941 cites W2027850807 @default.
• W2260851941 cites W2029069621 @default.
• W2260851941 cites W2029350545 @default.
• W2260851941 cites W2030204860 @default.
• W2260851941 cites W2031196100 @default.
• W2260851941 cites W2032463086 @default.
• W2260851941 cites W2033765460 @default.
• W2260851941 cites W2034407708 @default.
• W2260851941 cites W2035350746 @default.
• W2260851941 cites W2035396237 @default.
• W2260851941 cites W2037911390 @default.
• W2260851941 cites W2039555480 @default.
• W2260851941 cites W2040382301 @default.
• W2260851941 cites W2040487081 @default.

• next