FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2260888904> ?p ?o ?g. }

• W2260888904 endingPage "40" @default.
• W2260888904 startingPage "33" @default.
• W2260888904 abstract "Granzymes are serine proteases involved in the induction of cell death against non-self cells. The enzymes differ in their primary substrate specificity and have one of four hydrolysis activities: tryptase, Asp-ase, Met-ase and chymase. Although granzyme genes have been isolated from several fishes, evidence for their involvement in cytotoxicity has not yet been reported. In the present study, we attempted to purify and characterize a fish granzyme involved in cytotoxicity using ginbuna crucian carp. The cytotoxicity of leukocytes was significantly inhibited by the serine protease inhibitor ''3, 4-dichloroisocoumarin''. In addition, we found that granzymeA-like activity (hydrolysis of Z-GPR-MCA) was inhibited by the same inhibitor and significantly enhanced by allo-antigen stimulation in vivo. Proteins from leukocyte extracts were subjected to two steps of chromatographic purification using benzamidine-Sepharose and SP-Sepharose. The molecular weight of the purified enzyme was estimated to be 26,900 Da by SDS-PAGE analysis. The purified enzyme displayed a Km of 220 μM, a Kcat of 21.7 sec(-1) and a Kcat/Km of 98,796 sec(-1) M(-1) with an optimal pH of 9.5 for the Z-GPR-MCA substrate. The protease was totally inhibited by serine protease inhibitors and showed granzymeA-like substrate specificity. Therefore, we conclude that the purified enzyme belongs to the mammalian granzymeA (EC 3.4.21.78) and appears to be involved in cytotoxicity in fish." @default.
• W2260888904 created "2016-06-24" @default.
• W2260888904 creator A5030337738 @default.
• W2260888904 creator A5041818914 @default.
• W2260888904 creator A5048891278 @default.
• W2260888904 creator A5070835930 @default.
• W2260888904 creator A5079556204 @default.
• W2260888904 date "2016-07-01" @default.
• W2260888904 modified "2023-09-25" @default.
• W2260888904 title "Purification and characterization of a fish granzymeA involved in cell-mediated immunity" @default.
• W2260888904 cites W1552665179 @default.
• W2260888904 cites W1571433223 @default.
• W2260888904 cites W1588224088 @default.
• W2260888904 cites W1594120159 @default.
• W2260888904 cites W1605937980 @default.
• W2260888904 cites W1809964664 @default.
• W2260888904 cites W1969824361 @default.
• W2260888904 cites W1973720984 @default.
• W2260888904 cites W1977356076 @default.
• W2260888904 cites W1986962105 @default.
• W2260888904 cites W1991104246 @default.
• W2260888904 cites W1992080559 @default.
• W2260888904 cites W2002012875 @default.
• W2260888904 cites W2005981959 @default.
• W2260888904 cites W2007631902 @default.
• W2260888904 cites W2013285332 @default.
• W2260888904 cites W2014227845 @default.
• W2260888904 cites W2018473508 @default.
• W2260888904 cites W2033407152 @default.
• W2260888904 cites W2045651199 @default.
• W2260888904 cites W2052002634 @default.
• W2260888904 cites W2054979333 @default.
• W2260888904 cites W2056839074 @default.
• W2260888904 cites W2058406709 @default.
• W2260888904 cites W2067949684 @default.
• W2260888904 cites W2068005505 @default.
• W2260888904 cites W2068505697 @default.
• W2260888904 cites W2085677599 @default.
• W2260888904 cites W2089907993 @default.
• W2260888904 cites W2107880476 @default.
• W2260888904 cites W2115099741 @default.
• W2260888904 cites W2121563199 @default.
• W2260888904 cites W2137884390 @default.
• W2260888904 cites W2152463640 @default.
• W2260888904 cites W2171377633 @default.
• W2260888904 cites W2414723915 @default.
• W2260888904 cites W4232534952 @default.
• W2260888904 doi "https://doi.org/10.1016/j.dci.2016.02.011" @default.
• W2260888904 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26872543" @default.
• W2260888904 hasPublicationYear "2016" @default.
• W2260888904 type Work @default.
• W2260888904 sameAs 2260888904 @default.
• W2260888904 citedByCount "7" @default.
• W2260888904 countsByYear W22608889042017 @default.
• W2260888904 countsByYear W22608889042018 @default.
• W2260888904 countsByYear W22608889042019 @default.
• W2260888904 countsByYear W22608889042021 @default.
• W2260888904 crossrefType "journal-article" @default.
• W2260888904 hasAuthorship W2260888904A5030337738 @default.
• W2260888904 hasAuthorship W2260888904A5041818914 @default.
• W2260888904 hasAuthorship W2260888904A5048891278 @default.
• W2260888904 hasAuthorship W2260888904A5070835930 @default.
• W2260888904 hasAuthorship W2260888904A5079556204 @default.
• W2260888904 hasBestOaLocation W22608889042 @default.
• W2260888904 hasConcept C109316439 @default.
• W2260888904 hasConcept C153911025 @default.
• W2260888904 hasConcept C181199279 @default.
• W2260888904 hasConcept C182220744 @default.
• W2260888904 hasConcept C183193105 @default.
• W2260888904 hasConcept C202751555 @default.
• W2260888904 hasConcept C2775883766 @default.
• W2260888904 hasConcept C2776062744 @default.
• W2260888904 hasConcept C2776714187 @default.
• W2260888904 hasConcept C2777807008 @default.
• W2260888904 hasConcept C2780865716 @default.
• W2260888904 hasConcept C2781378782 @default.
• W2260888904 hasConcept C41183919 @default.
• W2260888904 hasConcept C55493867 @default.
• W2260888904 hasConcept C56856141 @default.
• W2260888904 hasConcept C86803240 @default.
• W2260888904 hasConceptScore W2260888904C109316439 @default.
• W2260888904 hasConceptScore W2260888904C153911025 @default.
• W2260888904 hasConceptScore W2260888904C181199279 @default.
• W2260888904 hasConceptScore W2260888904C182220744 @default.
• W2260888904 hasConceptScore W2260888904C183193105 @default.
• W2260888904 hasConceptScore W2260888904C202751555 @default.
• W2260888904 hasConceptScore W2260888904C2775883766 @default.
• W2260888904 hasConceptScore W2260888904C2776062744 @default.
• W2260888904 hasConceptScore W2260888904C2776714187 @default.
• W2260888904 hasConceptScore W2260888904C2777807008 @default.
• W2260888904 hasConceptScore W2260888904C2780865716 @default.
• W2260888904 hasConceptScore W2260888904C2781378782 @default.
• W2260888904 hasConceptScore W2260888904C41183919 @default.
• W2260888904 hasConceptScore W2260888904C55493867 @default.
• W2260888904 hasConceptScore W2260888904C56856141 @default.
• W2260888904 hasConceptScore W2260888904C86803240 @default.
• W2260888904 hasFunder F4320324704 @default.
• W2260888904 hasLocation W22608889041 @default.

• next