FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2261279968> ?p ?o ?g. }

• W2261279968 endingPage "C135" @default.
• W2261279968 startingPage "C126" @default.
• W2261279968 abstract "The Na+-K+-ATPase is specifically inhibited by cardiac glycosides, some of which may also function as endogenous mammalian hormones. Previous studies using Xenopus oocytes, yeast cells, or purified isoforms demonstrated that affinities of various cardiac glycosides for three isoforms of the Na+-K+-ATPase (α1-α3β1) may differ, a finding with potential clinical implication. The present study investigates isoform selectivity and effects of cardiac glycosides on cultured mammalian cells under more physiological conditions. H1299 cells (non-small cell lung carcinoma) were engineered to express only one α-isoform (α1, α2, or α3) by combining stable transfection of isoforms and silencing endogenous α1. Cardiac glycoside binding was measured by displacement of bound 3H-ouabain. The experiments confirm moderate α1/α3:α2 selectivity of ouabain, moderate α2:α1 selectivity of digoxin, and enhanced α2:α1 selectivity of synthetic derivatives (Katz A, Tal DM, Heller D, Haviv H, Rabah B, Barkana Y, Marcovich AL, Karlish SJD. J Biol Chem 289: 21153-21162, 2014). Relative α2:α1 selectivity of digoxin vs. ouabain was also manifested by enhanced internalization of α2 in response to digoxin. Cellular proliferation assays of H1299 cells confirmed the patterns of α2:α1 selectivity for ouabain, digoxin, and a synthetic derivative and reveal a crucial role of surface pump density on sensitivity to cardiac glycosides. Because cardiac glycosides are being considered as drugs for treatment of cancer, effects of ouabain on proliferation of 12 cancer and noncancer cell lines, with variable plasma membrane expression of α1, have been tested. These demonstrated that sensitivity to ouabain indeed depends linearly on the plasma membrane surface density of Na+-K+-ATPase irrespective of status, malignant or nonmalignant." @default.
• W2261279968 created "2016-06-24" @default.
• W2261279968 creator A5026149715 @default.
• W2261279968 creator A5065267260 @default.
• W2261279968 creator A5082141342 @default.
• W2261279968 date "2015-07-15" @default.
• W2261279968 modified "2023-09-24" @default.
• W2261279968 title "Cardiac glycosides induced toxicity in human cells expressing α1-, α2-, or α3-isoforms of Na-K-ATPase" @default.
• W2261279968 cites W1531911812 @default.
• W2261279968 cites W1539469412 @default.
• W2261279968 cites W1540591510 @default.
• W2261279968 cites W1917324330 @default.
• W2261279968 cites W1966158578 @default.
• W2261279968 cites W1966594228 @default.
• W2261279968 cites W1966680761 @default.
• W2261279968 cites W1974940196 @default.
• W2261279968 cites W1981791469 @default.
• W2261279968 cites W1986375790 @default.
• W2261279968 cites W1989124419 @default.
• W2261279968 cites W1998379414 @default.
• W2261279968 cites W2004336074 @default.
• W2261279968 cites W2011811704 @default.
• W2261279968 cites W2065069615 @default.
• W2261279968 cites W2070358111 @default.
• W2261279968 cites W2073212979 @default.
• W2261279968 cites W2077470893 @default.
• W2261279968 cites W2084029566 @default.
• W2261279968 cites W2092331689 @default.
• W2261279968 cites W2109683567 @default.
• W2261279968 cites W2120070648 @default.
• W2261279968 cites W2120194191 @default.
• W2261279968 cites W2122508595 @default.
• W2261279968 cites W2124275925 @default.
• W2261279968 cites W2124632723 @default.
• W2261279968 cites W2128316406 @default.
• W2261279968 cites W2140644734 @default.
• W2261279968 cites W2140692754 @default.
• W2261279968 cites W2143617499 @default.
• W2261279968 cites W2143791670 @default.
• W2261279968 cites W2148306415 @default.
• W2261279968 cites W2157438829 @default.
• W2261279968 cites W2162006210 @default.
• W2261279968 cites W2342978219 @default.
• W2261279968 cites W2772176708 @default.
• W2261279968 doi "https://doi.org/10.1152/ajpcell.00089.2015" @default.
• W2261279968 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25994790" @default.
• W2261279968 hasPublicationYear "2015" @default.
• W2261279968 type Work @default.
• W2261279968 sameAs 2261279968 @default.
• W2261279968 citedByCount "25" @default.
• W2261279968 countsByYear W22612799682016 @default.
• W2261279968 countsByYear W22612799682017 @default.
• W2261279968 countsByYear W22612799682018 @default.
• W2261279968 countsByYear W22612799682019 @default.
• W2261279968 countsByYear W22612799682020 @default.
• W2261279968 countsByYear W22612799682021 @default.
• W2261279968 countsByYear W22612799682022 @default.
• W2261279968 countsByYear W22612799682023 @default.
• W2261279968 crossrefType "journal-article" @default.
• W2261279968 hasAuthorship W2261279968A5026149715 @default.
• W2261279968 hasAuthorship W2261279968A5065267260 @default.
• W2261279968 hasAuthorship W2261279968A5082141342 @default.
• W2261279968 hasConcept C104317684 @default.
• W2261279968 hasConcept C118792377 @default.
• W2261279968 hasConcept C126322002 @default.
• W2261279968 hasConcept C161790260 @default.
• W2261279968 hasConcept C178790620 @default.
• W2261279968 hasConcept C185592680 @default.
• W2261279968 hasConcept C194671627 @default.
• W2261279968 hasConcept C2777707475 @default.
• W2261279968 hasConcept C2778198053 @default.
• W2261279968 hasConcept C2779133857 @default.
• W2261279968 hasConcept C2780557390 @default.
• W2261279968 hasConcept C53345823 @default.
• W2261279968 hasConcept C537181965 @default.
• W2261279968 hasConcept C54355233 @default.
• W2261279968 hasConcept C55493867 @default.
• W2261279968 hasConcept C71240020 @default.
• W2261279968 hasConcept C71924100 @default.
• W2261279968 hasConcept C81885089 @default.
• W2261279968 hasConcept C86803240 @default.
• W2261279968 hasConceptScore W2261279968C104317684 @default.
• W2261279968 hasConceptScore W2261279968C118792377 @default.
• W2261279968 hasConceptScore W2261279968C126322002 @default.
• W2261279968 hasConceptScore W2261279968C161790260 @default.
• W2261279968 hasConceptScore W2261279968C178790620 @default.
• W2261279968 hasConceptScore W2261279968C185592680 @default.
• W2261279968 hasConceptScore W2261279968C194671627 @default.
• W2261279968 hasConceptScore W2261279968C2777707475 @default.
• W2261279968 hasConceptScore W2261279968C2778198053 @default.
• W2261279968 hasConceptScore W2261279968C2779133857 @default.
• W2261279968 hasConceptScore W2261279968C2780557390 @default.
• W2261279968 hasConceptScore W2261279968C53345823 @default.
• W2261279968 hasConceptScore W2261279968C537181965 @default.
• W2261279968 hasConceptScore W2261279968C54355233 @default.
• W2261279968 hasConceptScore W2261279968C55493867 @default.
• W2261279968 hasConceptScore W2261279968C71240020 @default.
• W2261279968 hasConceptScore W2261279968C71924100 @default.

• next