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- W2261681736 abstract "This chapter describes the design and function of a pilot plant for the production of human fibroblast interferon in quantities of 100 to 200×106 units weekly from diploid cells or 400 to 800×106 units from a continuous cell line (MG-63). The system used is that originally designed for the production of high-titered rabbit interferon. This method, called superinduction, is subsequently adapted for use in human diploid fibroblasts. In a further study for production of fibroblast interferon by the tumor cell line MG-63 is presented. In developing the interferon production system, trial and error have led to use the bulk culture vessel (BCV)-device for large-scale propagation of fibroblasts, regular roller bottles for induction and production of interferon, and batch adsorption/desorption on controlled-pore glass (CPG) beads for concentration and partial purification. This routine method is applicable for production of interferon with diploid cells as well as with the osteosarcoma cell line, MG-63." @default.
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- W2261681736 date "1981-01-01" @default.
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- W2261681736 title "[13] Large-scale production of human fibroblast interferon" @default.
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- W2261681736 doi "https://doi.org/10.1016/0076-6879(81)78103-5" @default.
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