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• W2262458857 abstract "The primary functions of the proteasome are driven by a highly allosteric ATPase complex. ATP binding to only two subunits in this hexameric complex triggers substrate binding, ATPase-20S association and 20S gate opening. However, it is unclear how ATP binding and hydrolysis spatially and temporally coordinates these allosteric effects to drive substrate translocation into the 20S. Here, we use FRET to show that the proteasomal ATPases from eukaryotes (RPTs) and archaea (PAN) bind ATP with high affinity at neighbouring subunits, which complements the well-established spiral-staircase topology of the 26S ATPases. We further show that two conserved arginine fingers in PAN located at the subunit interface work together as a single allosteric unit to mediate the allosteric effects of ATP binding, without altering the nucleotide-binding pattern. Rapid kinetics analysis also shows that ring resetting of a sequential hydrolysis mechanism can be explained by thermodynamic equilibrium binding of ATP. These data support a model whereby these two functionally distinct allosteric networks cooperate to translocate polypeptides into the 20S for degradation." @default.
• W2262458857 created "2016-06-24" @default.
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• W2262458857 date "2015-10-14" @default.
• W2262458857 modified "2023-10-17" @default.
• W2262458857 title "ATP binding to neighbouring subunits and intersubunit allosteric coupling underlie proteasomal ATPase function" @default.
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• W2262458857 doi "https://doi.org/10.1038/ncomms9520" @default.
• W2262458857 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4608255" @default.
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• W2262458857 hasPublicationYear "2015" @default.
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