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• W2262610244 endingPage "14717" @default.
• W2262610244 startingPage "14714" @default.
• W2262610244 abstract "A new method for quantitative phopholipase activity assays using mass spectrometry (MS) and a supported thin film consisting of a graphene oxide (GO)/carbon nanotube (CNT) double layer as a substrate for laser desorption ionization (LDI) has been developed. Phospholipids were very efficiently analyzed by LDI−time-of-flight (TOF) MS on the GO/CNT films, presumably because of the affinity of phospholipids for the GO/CNT surface. Therefore, the rate of lipid hydrolysis was conveniently measured using LDI−TOF mass spectra obtained from a GO/CNT surface on which the phospholipid hydrolysis reaction mixtures had been spotted by comparing the mass-peak intensities of reactants and products. The present platform for phospholipase assays based on MS and GO/CNT double-layer films enables quantitative measurements at low cost, allows assays to be performed in a short time, and is compatible with an array format, unlike conventional assay methods." @default.
• W2262610244 created "2016-06-24" @default.
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• W2262610244 date "2010-10-01" @default.
• W2262610244 modified "2023-10-17" @default.
• W2262610244 title "Laser Desorption/Ionization Mass Spectrometric Assay for Phospholipase Activity Based on Graphene Oxide/Carbon Nanotube Double-Layer Films" @default.
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• W2262610244 doi "https://doi.org/10.1021/ja106276j" @default.
• W2262610244 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20886850" @default.
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