Matches in SemOpenAlex for { <https://semopenalex.org/work/W2262646480> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2262646480 endingPage "559" @default.
- W2262646480 startingPage "553" @default.
- W2262646480 abstract "Abstract We describe a modified method of analysis for total thyroxine in 50 µl of serum by equilibrium competitive protein binding with thyroxine-binding globulin. Columns (9 x 23 mm) are used containing Sephadex G-25, which are regenerated after each cycle by removing residual thyroxine adsorbed to the gel with excess thyroxine-binding globulin (dilute plasma). As little as 1 ng of hormone can be detected, and recoveries of added thyroxine from sera range from 98-102%. For either between-assay or within-assay determinations, the coefficients of variation are about 4%. Results of this method as compared with those for the uncorrected Murphy-Pattee procedure [J. Lab. Clin. Med. 66, 161 (1965)] gave a correlation coefficient of 0.944, but results with the present procedure are 11% higher because of incomplete and variable recovery with the uncorrected Murphy-Pattee method. Our procedure offers the advantage that, on a single column, thyroxine is quantitatively extracted from serum, rapidly brought to equilibrium binding with thyroxine binding globulin, and the free and bound thyroxine are separated. Cumbersome and lengthy procedures of extraction, centrifugation, and evaporation are thus obviated." @default.
- W2262646480 created "2016-06-24" @default.
- W2262646480 creator A5036520877 @default.
- W2262646480 creator A5086625441 @default.
- W2262646480 date "1974-05-01" @default.
- W2262646480 modified "2023-09-26" @default.
- W2262646480 title "Analysis for Total Serum Thyroxine by Equilibrium Competitive Protein Binding on Small, Re-usable Sephadex Columns" @default.
- W2262646480 doi "https://doi.org/10.1093/clinchem/20.5.553" @default.
- W2262646480 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4826948" @default.
- W2262646480 hasPublicationYear "1974" @default.
- W2262646480 type Work @default.
- W2262646480 sameAs 2262646480 @default.
- W2262646480 citedByCount "44" @default.
- W2262646480 countsByYear W22626464802012 @default.
- W2262646480 countsByYear W22626464802014 @default.
- W2262646480 crossrefType "journal-article" @default.
- W2262646480 hasAuthorship W2262646480A5036520877 @default.
- W2262646480 hasAuthorship W2262646480A5086625441 @default.
- W2262646480 hasBestOaLocation W22626464801 @default.
- W2262646480 hasConcept C134018914 @default.
- W2262646480 hasConcept C163994747 @default.
- W2262646480 hasConcept C170493617 @default.
- W2262646480 hasConcept C181199279 @default.
- W2262646480 hasConcept C185592680 @default.
- W2262646480 hasConcept C2777239931 @default.
- W2262646480 hasConcept C2778288131 @default.
- W2262646480 hasConcept C3019856697 @default.
- W2262646480 hasConcept C43617362 @default.
- W2262646480 hasConcept C55493867 @default.
- W2262646480 hasConcept C71315377 @default.
- W2262646480 hasConcept C71924100 @default.
- W2262646480 hasConcept C7623868 @default.
- W2262646480 hasConcept C81358767 @default.
- W2262646480 hasConcept C89838059 @default.
- W2262646480 hasConceptScore W2262646480C134018914 @default.
- W2262646480 hasConceptScore W2262646480C163994747 @default.
- W2262646480 hasConceptScore W2262646480C170493617 @default.
- W2262646480 hasConceptScore W2262646480C181199279 @default.
- W2262646480 hasConceptScore W2262646480C185592680 @default.
- W2262646480 hasConceptScore W2262646480C2777239931 @default.
- W2262646480 hasConceptScore W2262646480C2778288131 @default.
- W2262646480 hasConceptScore W2262646480C3019856697 @default.
- W2262646480 hasConceptScore W2262646480C43617362 @default.
- W2262646480 hasConceptScore W2262646480C55493867 @default.
- W2262646480 hasConceptScore W2262646480C71315377 @default.
- W2262646480 hasConceptScore W2262646480C71924100 @default.
- W2262646480 hasConceptScore W2262646480C7623868 @default.
- W2262646480 hasConceptScore W2262646480C81358767 @default.
- W2262646480 hasConceptScore W2262646480C89838059 @default.
- W2262646480 hasIssue "5" @default.
- W2262646480 hasLocation W22626464801 @default.
- W2262646480 hasLocation W22626464802 @default.
- W2262646480 hasOpenAccess W2262646480 @default.
- W2262646480 hasPrimaryLocation W22626464801 @default.
- W2262646480 hasRelatedWork W1935741213 @default.
- W2262646480 hasRelatedWork W1975215705 @default.
- W2262646480 hasRelatedWork W1983274469 @default.
- W2262646480 hasRelatedWork W2013477885 @default.
- W2262646480 hasRelatedWork W2050743802 @default.
- W2262646480 hasRelatedWork W2073496541 @default.
- W2262646480 hasRelatedWork W2099609559 @default.
- W2262646480 hasRelatedWork W2262646480 @default.
- W2262646480 hasRelatedWork W2347677701 @default.
- W2262646480 hasRelatedWork W2354860907 @default.
- W2262646480 hasVolume "20" @default.
- W2262646480 isParatext "false" @default.
- W2262646480 isRetracted "false" @default.
- W2262646480 magId "2262646480" @default.
- W2262646480 workType "article" @default.