FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2262765066> ?p ?o ?g. }

• W2262765066 abstract "Granzyme B (GrB) is a serine protease, traditionally known as expressed by cytotoxic lymphocytes to induce target cell apoptosis. However, it is emerging that GrB, being also produced by a variety of normal and neoplastic cells and potentially acting on multiple targets, might represent a powerful regulator of a wide range of fundamental biological processes. We have previously shown that GrB is expressed in urothelial carcinoma tissues and its expression is associated to both pathological tumor spreading and EMT. We have also shown that docosahexaenoic acid (DHA), a dietary ω-3 polyunsaturated fatty acid with anti-tumor activity, while inhibiting urothelial and pancreatic carcinoma cell invasion also inhibited their GrB expression in vitro. In this study, we characterized a panel of colorectal carcinoma (CRC) cells, with different invasive capabilities, for GrB expression and for the contribution of GrB to their EMT and invasive phenotype. In addition, we investigated the effect of DHA on CRC cell-associated GrB expression, EMT and invasion.The expression levels of GrB and EMT-related markers were evaluated by Western blotting. GrB knockdown was performed by Stealth RNAi small interfering RNA silencing and ectopic GrB expression by transfection of human GrB vector. Cell invasion was determined by the BioCoat Matrigel invasion chamber test.GrB was produced in 57.1% CRC cell lines and 100% CRC-derived Cancer Stem Cells. Although GrB was constitutive expressed in both invasive and noninvasive CRC cells, GrB depletion in invasive CRC cells downmodulated their invasion in vitro, suggesting a contribution of GrB to CRC invasiveness. GrB loss or gain of function downmodulated or upmodulated EMT, respectively, according to the analysis of cancer cell expression of three EMT biomarkers (Snail1, E-cadherin, N-cadherin). Moreover, TGF-β1-driven EMT was associated to the enhancement of GrB expression in CRC cell lines, and GrB depletion led to downmodulation of TGF-β1-driven EMT. In addition, DHA inhibited GrB expression, EMT and invasion in CRC cells in vitro.These findings present a novel role for GrB as upmodulator of EMT in CRC cells. Moreover, these results support the use of DHA, a dietary compound without toxic effects, as adjuvant in CRC therapy." @default.
• W2262765066 created "2016-06-24" @default.
• W2262765066 creator A5009420594 @default.
• W2262765066 creator A5022343395 @default.
• W2262765066 creator A5024871281 @default.
• W2262765066 creator A5025268176 @default.
• W2262765066 creator A5029693506 @default.
• W2262765066 creator A5040247962 @default.
• W2262765066 date "2016-02-02" @default.
• W2262765066 modified "2023-10-14" @default.
• W2262765066 title "Epitelial-to-mesenchimal transition and invasion are upmodulated by tumor-expressed granzyme B and inhibited by docosahexaenoic acid in human colorectal cancer cells" @default.
• W2262765066 cites W1516464304 @default.
• W2262765066 cites W1545985725 @default.
• W2262765066 cites W1598136252 @default.
• W2262765066 cites W1825962579 @default.
• W2262765066 cites W1964000096 @default.
• W2262765066 cites W1965574533 @default.
• W2262765066 cites W1984908915 @default.
• W2262765066 cites W1988784569 @default.
• W2262765066 cites W1990757940 @default.
• W2262765066 cites W1995275059 @default.
• W2262765066 cites W1999177893 @default.
• W2262765066 cites W2000537420 @default.
• W2262765066 cites W2000718958 @default.
• W2262765066 cites W2005779705 @default.
• W2262765066 cites W2012110635 @default.
• W2262765066 cites W2028577830 @default.
• W2262765066 cites W2029231546 @default.
• W2262765066 cites W2030938449 @default.
• W2262765066 cites W2032149953 @default.
• W2262765066 cites W2032245328 @default.
• W2262765066 cites W2033482909 @default.
• W2262765066 cites W2035122148 @default.
• W2262765066 cites W2037131199 @default.
• W2262765066 cites W2046853205 @default.
• W2262765066 cites W2048647257 @default.
• W2262765066 cites W2049491375 @default.
• W2262765066 cites W2057768232 @default.
• W2262765066 cites W2058481603 @default.
• W2262765066 cites W2063249351 @default.
• W2262765066 cites W2074604431 @default.
• W2262765066 cites W2074731906 @default.
• W2262765066 cites W2078776989 @default.
• W2262765066 cites W2095011910 @default.
• W2262765066 cites W2098940019 @default.
• W2262765066 cites W2099361912 @default.
• W2262765066 cites W2109832697 @default.
• W2262765066 cites W2113207478 @default.
• W2262765066 cites W2117214964 @default.
• W2262765066 cites W2121870966 @default.
• W2262765066 cites W2126629487 @default.
• W2262765066 cites W2128506942 @default.
• W2262765066 cites W2143468862 @default.
• W2262765066 cites W2144467454 @default.
• W2262765066 cites W2146389661 @default.
• W2262765066 cites W2149148649 @default.
• W2262765066 cites W2151855684 @default.
• W2262765066 cites W2153416327 @default.
• W2262765066 cites W2156826845 @default.
• W2262765066 cites W2158049101 @default.
• W2262765066 cites W2172002563 @default.
• W2262765066 cites W2325753456 @default.
• W2262765066 cites W235800868 @default.
• W2262765066 cites W2416347995 @default.
• W2262765066 cites W2917837889 @default.
• W2262765066 cites W613107733 @default.
• W2262765066 doi "https://doi.org/10.1186/s13046-016-0302-6" @default.
• W2262765066 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4736710" @default.
• W2262765066 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26830472" @default.
• W2262765066 hasPublicationYear "2016" @default.
• W2262765066 type Work @default.
• W2262765066 sameAs 2262765066 @default.
• W2262765066 citedByCount "32" @default.
• W2262765066 countsByYear W22627650662016 @default.
• W2262765066 countsByYear W22627650662017 @default.
• W2262765066 countsByYear W22627650662018 @default.
• W2262765066 countsByYear W22627650662019 @default.
• W2262765066 countsByYear W22627650662020 @default.
• W2262765066 countsByYear W22627650662021 @default.
• W2262765066 countsByYear W22627650662022 @default.
• W2262765066 countsByYear W22627650662023 @default.
• W2262765066 crossrefType "journal-article" @default.
• W2262765066 hasAuthorship W2262765066A5009420594 @default.
• W2262765066 hasAuthorship W2262765066A5022343395 @default.
• W2262765066 hasAuthorship W2262765066A5024871281 @default.
• W2262765066 hasAuthorship W2262765066A5025268176 @default.
• W2262765066 hasAuthorship W2262765066A5029693506 @default.
• W2262765066 hasAuthorship W2262765066A5040247962 @default.
• W2262765066 hasBestOaLocation W22627650661 @default.
• W2262765066 hasConcept C173396325 @default.
• W2262765066 hasConcept C203014093 @default.
• W2262765066 hasConcept C2776090121 @default.
• W2262765066 hasConcept C2780380082 @default.
• W2262765066 hasConcept C502942594 @default.
• W2262765066 hasConcept C54355233 @default.
• W2262765066 hasConcept C81885089 @default.
• W2262765066 hasConcept C86803240 @default.
• W2262765066 hasConcept C8891405 @default.
• W2262765066 hasConceptScore W2262765066C173396325 @default.
• W2262765066 hasConceptScore W2262765066C203014093 @default.

• next