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- W2262907345 abstract "Objective To construct a lentiviral vector expressing microRNA,hsa-miR-30e.Methods Pri-miR-30e amplified by PCR was inserted into pll3.7 vector,and then confirmed by restriction endonuclease analysis and DNA sequencing.Then 293FT cells were contransfected with recombinant plasmid and helper plasmid.The expression of miR-30e was detected by fluorescence and Real-time PCR.Result The recombinant plasmid contains the target sequence of miR-30e and can express GFP successfully.Conclusion The constructed lentiviral vector can express miR-30e in vitro,providing the fundation for further study of miR-30e." @default.
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- W2262907345 date "2010-01-01" @default.
- W2262907345 modified "2023-09-24" @default.
- W2262907345 title "Construction of recombinant lentiviral vector expressing hsa-miR-30e." @default.
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