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- W2263293603 abstract "1152 In order to identify molecules involved in pulmonary carcinogenesis and those to be useful as diagnostic markers as well as for development of novel drugs and immunotherapy, we established a screening system by a combination of the cDNA microarray analysis and experiments using antisense S-oligonucleotide, RNA interference (RNAi) technique, and tumor tissue microarray. We performed a cDNA microarray representing 27,648 genes for 37 non-small cell lung cancer cells (NSCLCs) purified by laser-capture microdissection and selected 428 candidate genes as tumor markers or therapeutic targets, which are specifically expressed in NSCLCs, but not in vital tissues. We then determined the full-length sequences of the 328 genes by EST screening and confirmed their gene expression pattern using semi-quantitative RT-PCR and/or northern-blot analyses. To assess whether each of these genes plays a role in the growth or survival of lung-cancer cells, we applied the antisense S-oligonucleotide and RNAi technique, and identified several genes that could be good candidates for the development of novel therapeutic drugs or immunotherapy. These genes encode proteins that enhanced cell growth, some in an autocrine manner. In addition to them, we also identified cytoplasmic membrane proteins that are likely to be candidate tumor markers for NSCLC. Subsequent analysis detected high levels of individual proteins in serum from NSCLC patients using ELISA. We also correlated their expression patterns with clinicopathological data, including prognosis by immunohistochemistry of tumor tissue microarrays in 400 archived NSCLCs. Our results, obtained by the combined analyses of the high throughput approaches to determine gene expression profiles, their oncogenic functions, and their correlation with the clinical phenotype, could provide valuable information that will help us to eventually identify novel therapeutic target molecules and predictive markers for this type of cancer." @default.
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- W2263293603 date "2004-04-01" @default.
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- W2263293603 title "Topoisomerase II alpha expression as a predictive marker in a population of advanced breast cancer patients randomly treated with single-agent doxorubicin or single-agent docetaxel" @default.
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