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• W2264165890 abstract "A 35-year-old female patient from Waterloo, Ontario was diagnosed with pulmonary tuberculosis in June 1995. Records indicated that the patient had emigrated from Laos circa 1990. A culture grown from a bronchoalveolar lavage specimen was identified as Mycobacterium tuberculosis by standard biochemical methods. Drug-susceptibility testing indicated the strain was resistant to pyrazinamide (PZA), and a mutation was detected within pncA , a gene associated with PZA resistance. Sequence data from the 16S rRNA gene and the 16S/23S rRNA gene spacer confirmed that the strain was a member of the M tuberculosis complex, and analysis of the mpcA and pncA genes supported the identification of the strain as M tuberculosis rather than Mycobacterium bovis . However, the insertion element IS 6110 , which is used for epidemiological tracing of M tuberculosis , was not detected in this strain by either restriction fragment length polymorphism analysis or by polymerase chain reaction. Two other genetic markers associated with the M tuberculosis complex, IS 1081 and the direct repeat element, were present. The arrival of immigrants with tuberculosis from southeast Asia, where most strains of M tuberculosis lacking IS 6110 have been traced, has important implications for epidemiological studies of tuberculosis in North America." @default.
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• W2264165890 date "1998-01-01" @default.
• W2264165890 modified "2023-09-27" @default.
• W2264165890 title "Absence of the Genetic Marker IS6110from a Strain ofMycobacterium tuberculosisIsolated in Ontario" @default.
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• W2264165890 doi "https://doi.org/10.1155/1998/292491" @default.
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