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- W2264218001 abstract "To determine whether DNA vaccine plasmid could be expressed in eukaryotic cells and to explore whether the plasmid could be integrated into host genome,Toxoplasma gondii DNA vaccine pcDNA3.1-GRA6 was took as an example to establish identification methods of DNA recombinant vaccines both in vitro and in vivo.Using pcDNA3.1-EYFP as the control plasmid in vitro,yellow fluorescent protein could be expressed in eukaryotic cells.The best condition for transfection was the mixture of the plasmid DNA with EntransterTM-D mixed at the ratio of 1∶8.Then the expression of GRA6 protein from pcDNA3.1-GRA6 in the transfected cells was validated by RT-PCR and Western-blot analyses.For in vivo assay,PCR was taken to detect the distribution of plasmid DNA in mice at different time points post-immunization on day 28 post-last-immunization,GRA6 gene could be amplified from DNA samples extracted from the spleen,liver and other tissues of the immunized mice,while on day 56 post-immunization,GRA6 gene could not be amplified from all these organs,suggesting that plasmid DNA did not be integrated into the host genome.The establishment of expression and identification methods for DNA vaccine in vitro and in vivo laid the foundation for development of DNA vaccines." @default.
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- W2264218001 date "2010-01-01" @default.
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- W2264218001 title "Establishment of expression and identification methods for DNA vaccine in vitro and in vivo" @default.
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