FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2264340267> ?p ?o ?g. }

• W2264340267 endingPage "2330" @default.
• W2264340267 startingPage "2319" @default.
• W2264340267 abstract "Epiregulin (EPR) is a ligand of the epidermal growth factor (EGF) family that upon binding to its epidermal growth factor receptor (EGFR) stimulates proliferative signaling, especially in colon cancer cells. Here, we describe the three-dimensional structure of the EPR antibody (the 9E5(Fab) fragment) in the presence and absence of EPR. Among the six complementarity-determining regions (CDRs), CDR1–3 in the light chain and CDR2 in the heavy chain predominantly recognize EPR. In particular, CDR3 in the heavy chain dramatically moves with cis-trans isomerization of Pro103. A molecular dynamics simulation and mutational analyses revealed that Arg40 in EPR is a key residue for the specific binding of 9E5 IgG. From isothermal titration calorimetry analysis, the dissociation constant was determined to be 6.5 nm. Surface plasmon resonance analysis revealed that the dissociation rate of 9E5 IgG is extremely slow. The superimposed structure of 9E5(Fab)·EPR on the known complex structure of EGF·EGFR showed that the 9E5(Fab) paratope overlaps with Domains I and III on the EGFR, which reveals that the 9E5(Fab)·EPR complex could not bind to the EGFR. The 9E5 antibody will also be useful in medicine as a neutralizing antibody specific for colon cancer." @default.
• W2264340267 created "2016-06-24" @default.
• W2264340267 creator A5002191988 @default.
• W2264340267 creator A5004349096 @default.
• W2264340267 creator A5011709604 @default.
• W2264340267 creator A5014010927 @default.
• W2264340267 creator A5022405108 @default.
• W2264340267 creator A5026341150 @default.
• W2264340267 creator A5032712562 @default.
• W2264340267 creator A5040138257 @default.
• W2264340267 creator A5041893900 @default.
• W2264340267 creator A5048073780 @default.
• W2264340267 creator A5053908732 @default.
• W2264340267 creator A5063358671 @default.
• W2264340267 creator A5066735781 @default.
• W2264340267 creator A5071116943 @default.
• W2264340267 creator A5072979871 @default.
• W2264340267 creator A5076350850 @default.
• W2264340267 creator A5084105797 @default.
• W2264340267 creator A5085184505 @default.
• W2264340267 date "2016-01-01" @default.
• W2264340267 modified "2023-10-15" @default.
• W2264340267 title "Epiregulin Recognition Mechanisms by Anti-epiregulin Antibody 9E5" @default.
• W2264340267 cites W1491713991 @default.
• W2264340267 cites W1548640328 @default.
• W2264340267 cites W1834492561 @default.
• W2264340267 cites W1964321121 @default.
• W2264340267 cites W1966078827 @default.
• W2264340267 cites W1966658428 @default.
• W2264340267 cites W1974024530 @default.
• W2264340267 cites W1986137370 @default.
• W2264340267 cites W1991794210 @default.
• W2264340267 cites W1994655768 @default.
• W2264340267 cites W1996607909 @default.
• W2264340267 cites W2012322739 @default.
• W2264340267 cites W2017639307 @default.
• W2264340267 cites W2025986113 @default.
• W2264340267 cites W2041638382 @default.
• W2264340267 cites W2043792125 @default.
• W2264340267 cites W2058620780 @default.
• W2264340267 cites W2059521934 @default.
• W2264340267 cites W2067174909 @default.
• W2264340267 cites W2072219853 @default.
• W2264340267 cites W2074986801 @default.
• W2264340267 cites W2081693079 @default.
• W2264340267 cites W2083445427 @default.
• W2264340267 cites W2083831897 @default.
• W2264340267 cites W2092769453 @default.
• W2264340267 cites W2121805508 @default.
• W2264340267 cites W2124712330 @default.
• W2264340267 cites W2127322768 @default.
• W2264340267 cites W2128572087 @default.
• W2264340267 cites W2134201060 @default.
• W2264340267 cites W2137750974 @default.
• W2264340267 cites W2144081223 @default.
• W2264340267 cites W2150580590 @default.
• W2264340267 cites W2154197653 @default.
• W2264340267 cites W2159211495 @default.
• W2264340267 cites W2160544821 @default.
• W2264340267 cites W2171268876 @default.
• W2264340267 doi "https://doi.org/10.1074/jbc.m115.656009" @default.
• W2264340267 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4732215" @default.
• W2264340267 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26627827" @default.
• W2264340267 hasPublicationYear "2016" @default.
• W2264340267 type Work @default.
• W2264340267 sameAs 2264340267 @default.
• W2264340267 citedByCount "11" @default.
• W2264340267 countsByYear W22643402672016 @default.
• W2264340267 countsByYear W22643402672017 @default.
• W2264340267 countsByYear W22643402672019 @default.
• W2264340267 countsByYear W22643402672020 @default.
• W2264340267 countsByYear W22643402672021 @default.
• W2264340267 countsByYear W22643402672022 @default.
• W2264340267 crossrefType "journal-article" @default.
• W2264340267 hasAuthorship W2264340267A5002191988 @default.
• W2264340267 hasAuthorship W2264340267A5004349096 @default.
• W2264340267 hasAuthorship W2264340267A5011709604 @default.
• W2264340267 hasAuthorship W2264340267A5014010927 @default.
• W2264340267 hasAuthorship W2264340267A5022405108 @default.
• W2264340267 hasAuthorship W2264340267A5026341150 @default.
• W2264340267 hasAuthorship W2264340267A5032712562 @default.
• W2264340267 hasAuthorship W2264340267A5040138257 @default.
• W2264340267 hasAuthorship W2264340267A5041893900 @default.
• W2264340267 hasAuthorship W2264340267A5048073780 @default.
• W2264340267 hasAuthorship W2264340267A5053908732 @default.
• W2264340267 hasAuthorship W2264340267A5063358671 @default.
• W2264340267 hasAuthorship W2264340267A5066735781 @default.
• W2264340267 hasAuthorship W2264340267A5071116943 @default.
• W2264340267 hasAuthorship W2264340267A5072979871 @default.
• W2264340267 hasAuthorship W2264340267A5076350850 @default.
• W2264340267 hasAuthorship W2264340267A5084105797 @default.
• W2264340267 hasAuthorship W2264340267A5085184505 @default.
• W2264340267 hasBestOaLocation W22643402671 @default.
• W2264340267 hasConcept C104317684 @default.
• W2264340267 hasConcept C106847996 @default.
• W2264340267 hasConcept C121332964 @default.
• W2264340267 hasConcept C12554922 @default.
• W2264340267 hasConcept C153911025 @default.

• next