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• W2264754619 abstract "In this study, several RNA polymerases were used for the first time to examine the possibility of transcriptional incorporation of 5'-N-triphosphates of 5'-amino-5'-deoxyribonucleosides (5'NH NTPs). The T3, T7, Sp6 and T7 Y639F RNA polymerases were employed to show that the full-length transcript cannot be synthesized. The results suggest that the application of 5'NH NTPs could decrease transcription reaction rates. What is more, the modification of transcription conditions had no influence on the rate of 5'NH NTPs incorporation. Based on experimental data it is postulated that 5'NH NTPs can be used as potential transcription inhibitors. Our findings expand the knowledge on suitable uses of the 5'-N-triphosphates of 5'-amino-5'-deoxyribonucleoside and the exact mechanism of transcriptional inhibition." @default.
• W2264754619 created "2016-06-24" @default.
• W2264754619 creator A5078966154 @default.
• W2264754619 creator A5080671587 @default.
• W2264754619 creator A5091860223 @default.
• W2264754619 date "2016-02-01" @default.
• W2264754619 modified "2023-09-25" @default.
• W2264754619 title "Studies on Transcriptional Incorporation of 5’-N-Triphosphates of 5’-Amino-5’-Deoxyribonucleosides" @default.
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• W2264754619 doi "https://doi.org/10.1371/journal.pone.0148282" @default.
• W2264754619 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4735469" @default.
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• W2264754619 hasPublicationYear "2016" @default.
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