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• W2264824142 abstract "ABDJAD ASIH NAWANGSIH. Selection and caracterization of biocontrol bacteria to control the bacterial wilt disease (Ralstonia solanacearum) of tomato. Supervised by BUDI TJAHJONO, ANTONIUS SUWANTO, MEITY SURADJI SINAGA dan GUSTAF ADOLF WATTIMENA. Bacterial wilt disease caused by Ralstonia solanacearum is one of the important disease on some comodities in tropical, subtropical and warmer area in the world. The disease is important not only because of the geografic distribution, but also because of its wide host range. Application of some antibiotics will caused of resistance. Disease control using the resistant varieties was difficult because the pathogen have some races. The other alternatives for disease control is the application of biocontrol agents. In this experiments the biocontrol agents were isolated from the rhizosphere of healthy tomato among the one infected by tomato bacterial wilt. The isolates of biocontrol agents were investigated of their suppression to R. solanacearum by antibiosis mechanism in some agar media and their competitiveness in King’s B broth medium. From these experiment, three isolates were found potential to suppress the population of R. solanacearum. Identification based on the fisiological characteristics and partial sequence of 16S rDNA, the isolate RH4003 was identified as Pseudomonas fluorescens RH4003 while isolate L32 was identified as B. cereus L32. Control effectiveness in the green house of P.fluorescens RH4003 or B. cereus L32 was up to 62% while in the open field control effectiveness of B. subtilis AB89, P. fluorescens RH4003 and B. cereus L32 was up to 35, 32 and 22%, respectively. All of the biocontrol agents tested have no phytotoxic effects on seed viability and the growth of tomato seedlings and the population of biocontrol agents was still high, i.e. ± 10 cfu/gram root until 24 days after application into the soil. P. Fluorescens RH4003, B. subtilis AB89 and B. cereus L32 were able to induced the host defence by increasing the activity of peroxidase in the root. The bacteria were also produce siderophore. B. subtilis AB89 and B. cereus L32 produced protease but P. fluorescens RH4003 was not. All of the biocontrol agents did not produce chitinase and HCN. P. fluorescens RH4003 applied as seed treatment, soil infestation and combination both of them gave the highest suppression to the disease compared with B. subtilis AB89 or B. cereus L32. Based on the Synergy Factor (SF) value, combination among the biocontrol agents resulted in antagonistic relationship. P. fluorescens RH4003 relatively effective to control the bacterial wilt disease on the relative susceptible varieties (Money Maker and San Marzano) compared with the relatively resistant ones (Ratna and TM39). Total colonies of P. fluorescens RH4003 isolated from the root of Ratna variety were relatively higher than on the other tomato varieties. Judul Disertasi : Seleksi dan Karakterisasi Bakteri Biokontrol untuk Mengandalikan Penyakit Layu Bakteri (Ralstonia solanacearum) pada Tomat Nama : Abdjad Asih Nawangsih NIM : P 086 00003" @default.
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• W2264824142 title "Seleksi dan karakterisasi bakteri biokontrol untuk mengendalikan penyakit layu bakteri (Ralstonia solanacearum) pada tomat" @default.
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