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- W2266569897 endingPage "e0135215" @default.
- W2266569897 startingPage "e0135215" @default.
- W2266569897 abstract "Thermal induction of parthenogenesis (also known as thermal parthenogenesis) in silkworms is an important technique that has been used in artificial insemination, expansion of hybridization, transgenesis and sericultural production; however, the exact mechanisms of this induction remain unclear. This study aimed to investigate the gene expression profile in silkworms undergoing thermal parthenogenesis using RNA-seq analysis. The transcriptome profiles indicated that in non-induced and induced eggs, the numbers of differentially expressed genes (DEGs) for the parthenogenetic line (PL) and amphigenetic line (AL) were 538 and 545, respectively, as determined by fold-change ≥ 2. Gene ontology (GO) analysis showed that DEGs between two lines were mainly involved in reproduction, formation of chorion, female gamete generation and cell development pathways. Upregulation of many chorion genes in AL suggests that the maturation rate of AL eggs was slower than PL eggs. Some DEGs related to reactive oxygen species removal, DNA repair and heat shock response were differentially expressed between the two lines, such as MPV-17, REV1 and HSP68. These results supported the view that a large fraction of genes are differentially expressed between PL and AL, which offers a new approach to identifying the molecular mechanism of silkworm thermal parthenogenesis." @default.
- W2266569897 created "2016-06-24" @default.
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- W2266569897 date "2015-08-14" @default.
- W2266569897 modified "2023-10-16" @default.
- W2266569897 title "Transcriptome Analysis of Thermal Parthenogenesis of the Domesticated Silkworm" @default.
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- W2266569897 doi "https://doi.org/10.1371/journal.pone.0135215" @default.
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