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- W2267335819 abstract "Biogas production is an economically attractive technology that has gained momentum worldwide over the past years. Biogas is produced by a biologically mediated process, widely known as “anaerobic digestion.” This process is performed by a specialized and complex microbial community, in which different members have distinct roles in the establishment of a collective organization. Deciphering the complex microbial community engaged in this process is interesting both for unraveling the network of bacterial interactions and for applicability potential to the derived knowledge. In this study, we dissect the bioma involved in anaerobic digestion by means of high throughput Illumina sequencing (~51 gigabases of sequence data), disclosing nearly one million genes and extracting 106 microbial genomes by a novel strategy combining two binning processes. Microbial phylogeny and putative taxonomy performed using >400 proteins revealed that the biogas community is a trove of new species. A new approach based on functional properties as per network representation was developed to assign roles to the microbial species. The organization of the anaerobic digestion microbiome is resembled by a funnel concept, in which the microbial consortium presents a progressive functional specialization while reaching the final step of the process (i.e., methanogenesis). Key microbial genomes encoding enzymes involved in specific metabolic pathways, such as carbohydrates utilization, fatty acids degradation, amino acids fermentation, and syntrophic acetate oxidation, were identified. Additionally, the analysis identified a new uncultured archaeon that was putatively related to Methanomassiliicoccales but surprisingly having a methylotrophic methanogenic pathway. This study is a pioneer research on the phylogenetic and functional characterization of the microbial community populating biogas reactors. By applying for the first time high-throughput sequencing and a novel binning strategy, the identified genes were anchored to single genomes providing a clear understanding of their metabolic pathways and highlighting their involvement in anaerobic digestion. The overall research established a reference catalog of biogas microbial genomes that will greatly simplify future genomic studies." @default.
- W2267335819 created "2016-06-24" @default.
- W2267335819 creator A5005828835 @default.
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- W2267335819 date "2016-02-02" @default.
- W2267335819 modified "2023-10-06" @default.
- W2267335819 title "Metagenomic analysis and functional characterization of the biogas microbiome using high throughput shotgun sequencing and a novel binning strategy" @default.
- W2267335819 cites W1431942900 @default.
- W2267335819 cites W1483776899 @default.
- W2267335819 cites W1584790813 @default.
- W2267335819 cites W1586483823 @default.
- W2267335819 cites W1593751564 @default.
- W2267335819 cites W1672900003 @default.
- W2267335819 cites W1969346416 @default.
- W2267335819 cites W1970443385 @default.
- W2267335819 cites W1979006961 @default.
- W2267335819 cites W1986372069 @default.
- W2267335819 cites W1989348205 @default.
- W2267335819 cites W1995692625 @default.
- W2267335819 cites W2001561488 @default.
- W2267335819 cites W2001593327 @default.
- W2267335819 cites W2004123938 @default.
- W2267335819 cites W2004626381 @default.
- W2267335819 cites W2013935032 @default.
- W2267335819 cites W2018931571 @default.
- W2267335819 cites W2019355751 @default.
- W2267335819 cites W2027819084 @default.
- W2267335819 cites W2027847170 @default.
- W2267335819 cites W2032792259 @default.
- W2267335819 cites W2041209538 @default.
- W2267335819 cites W2043148175 @default.
- W2267335819 cites W2049702154 @default.
- W2267335819 cites W2065523323 @default.
- W2267335819 cites W2065775222 @default.
- W2267335819 cites W2065848015 @default.
- W2267335819 cites W2068536623 @default.
- W2267335819 cites W2071537506 @default.
- W2267335819 cites W2078078867 @default.
- W2267335819 cites W2083297018 @default.
- W2267335819 cites W2083395367 @default.
- W2267335819 cites W2085926377 @default.
- W2267335819 cites W2088521358 @default.
- W2267335819 cites W2089859322 @default.
- W2267335819 cites W2102619694 @default.
- W2267335819 cites W2103481476 @default.
- W2267335819 cites W2103901746 @default.
- W2267335819 cites W2104591221 @default.
- W2267335819 cites W2108383788 @default.
- W2267335819 cites W2108682145 @default.
- W2267335819 cites W2109654176 @default.
- W2267335819 cites W2110300022 @default.
- W2267335819 cites W2112364185 @default.
- W2267335819 cites W2113912602 @default.
- W2267335819 cites W2116711423 @default.
- W2267335819 cites W2116913446 @default.
- W2267335819 cites W2121635440 @default.
- W2267335819 cites W2122760303 @default.
- W2267335819 cites W2127862315 @default.
- W2267335819 cites W2131271579 @default.
- W2267335819 cites W2131848047 @default.
- W2267335819 cites W2133579817 @default.
- W2267335819 cites W2135621733 @default.
- W2267335819 cites W2135875463 @default.
- W2267335819 cites W2136032284 @default.
- W2267335819 cites W2136632285 @default.
- W2267335819 cites W2139816774 @default.
- W2267335819 cites W2141202455 @default.
- W2267335819 cites W2141766130 @default.
- W2267335819 cites W2142428670 @default.
- W2267335819 cites W2147526198 @default.
- W2267335819 cites W2150208009 @default.
- W2267335819 cites W2152885278 @default.
- W2267335819 cites W2153177488 @default.
- W2267335819 cites W2157107905 @default.
- W2267335819 cites W2157220051 @default.
- W2267335819 cites W2159406441 @default.
- W2267335819 cites W2159591897 @default.
- W2267335819 cites W2162686769 @default.
- W2267335819 cites W2162863397 @default.
- W2267335819 cites W2163089766 @default.
- W2267335819 cites W2166123725 @default.
- W2267335819 cites W2168330474 @default.
- W2267335819 cites W2168830117 @default.
- W2267335819 cites W2170551349 @default.
- W2267335819 cites W2285998378 @default.
- W2267335819 cites W2324727937 @default.
- W2267335819 cites W4250921295 @default.
- W2267335819 cites W4253720657 @default.
- W2267335819 cites W45534775 @default.
- W2267335819 doi "https://doi.org/10.1186/s13068-016-0441-1" @default.
- W2267335819 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4736482" @default.
- W2267335819 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26839589" @default.
- W2267335819 hasPublicationYear "2016" @default.
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