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- W2267504107 abstract "Objective To investigate the effect of 5FU on colorectal cancer cell lines SW480 and clarify the role of Musashi-1 in the effect.Methods IC50(50% inhibiting concentration)of 5-FU was measured by MTT assay.The proportion of SP cells and CD34+CD44+cells were obtained by flow cytometry(FCM).The expressions of Musashi-1 mRNA was detected by reverse transcription-polymerase chain reaction(RT-PCR).Results The IC50 of 5-FU at different time point as 24,48 and 72 h was 32.12、16.2 and 6.8 mg·L-1 respectively.Compared with normal control group,the proportion of SP cells was increased in experimental groups,as well as the proportion of CD34+CD44+ cells and expressions of Musashi-1 mRNA.Conclusion Musashi-1 may become one of markers for cancer stem cells in colorectal cancer cells.CD34 and CD44 have silimar potentials." @default.
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- W2267504107 date "2007-01-01" @default.
- W2267504107 modified "2023-09-23" @default.
- W2267504107 title "Expression of Musashi-1 gene:the effect of 5-FU on SW480 cell line" @default.
- W2267504107 hasPublicationYear "2007" @default.
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