Matches in SemOpenAlex for { <https://semopenalex.org/work/W2268682485> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2268682485 endingPage "569" @default.
- W2268682485 startingPage "563" @default.
- W2268682485 abstract "All organisms with oxygenic photosynthesis contain two photosystems: photosystemⅠ(PSⅠ) and photosystem-Ⅱ-(PSⅡ). The minimal photosystem-Ⅱ-particles which are photochemically active contain three subunits: D1, D2 and cytochrome b559 (Cyt b559). The function of Cyt b559 remains unclear. We have successfully overexpressed the psbF gene, encoding the - subunit of Cyt b559, from a marine cyanobacterium Synechococcus sp. PCC 7002 as a fusion gene and obtained a redox-active form of Cyt b559. When the N-terminal GST protein of the fusion gene product was removed with thrombin, the PsbF protein was still redox-active, suggesting that the recombinant PsbF can form dimer in Escherichia coli. The absorption spectra of either the oxidized form or the reduced form of both GST fusion protein and the purified PsbF dimer and the difference spectra between the two forms are the same as that of the Cyt b559 isolated from the higher plants. Redox titration analysis of recombinant PsbF showed that the mid-point redox potential of the recombinant Cyt b559 was approximately 50 mV, which is close to the low potential of Cyt b559. The results are helpful to the understanding of localization and function of Cyt b559 on thylakoid membranes." @default.
- W2268682485 created "2016-06-24" @default.
- W2268682485 creator A5046682092 @default.
- W2268682485 creator A5047413755 @default.
- W2268682485 date "2003-01-01" @default.
- W2268682485 modified "2023-09-23" @default.
- W2268682485 title "Recombinant PsbF from Synechococcus sp.PCC 7002 forms β：βhomodimeric cytochrome b559" @default.
- W2268682485 hasPublicationYear "2003" @default.
- W2268682485 type Work @default.
- W2268682485 sameAs 2268682485 @default.
- W2268682485 citedByCount "0" @default.
- W2268682485 crossrefType "journal-article" @default.
- W2268682485 hasAuthorship W2268682485A5046682092 @default.
- W2268682485 hasAuthorship W2268682485A5047413755 @default.
- W2268682485 hasConcept C154828652 @default.
- W2268682485 hasConcept C181199279 @default.
- W2268682485 hasConcept C183688256 @default.
- W2268682485 hasConcept C185592680 @default.
- W2268682485 hasConcept C2780768313 @default.
- W2268682485 hasConcept C55493867 @default.
- W2268682485 hasConcept C75297921 @default.
- W2268682485 hasConcept C80298142 @default.
- W2268682485 hasConcept C86803240 @default.
- W2268682485 hasConceptScore W2268682485C154828652 @default.
- W2268682485 hasConceptScore W2268682485C181199279 @default.
- W2268682485 hasConceptScore W2268682485C183688256 @default.
- W2268682485 hasConceptScore W2268682485C185592680 @default.
- W2268682485 hasConceptScore W2268682485C2780768313 @default.
- W2268682485 hasConceptScore W2268682485C55493867 @default.
- W2268682485 hasConceptScore W2268682485C75297921 @default.
- W2268682485 hasConceptScore W2268682485C80298142 @default.
- W2268682485 hasConceptScore W2268682485C86803240 @default.
- W2268682485 hasIssue "6" @default.
- W2268682485 hasLocation W22686824851 @default.
- W2268682485 hasOpenAccess W2268682485 @default.
- W2268682485 hasPrimaryLocation W22686824851 @default.
- W2268682485 hasRelatedWork W1540747875 @default.
- W2268682485 hasRelatedWork W1573638450 @default.
- W2268682485 hasRelatedWork W1994270259 @default.
- W2268682485 hasRelatedWork W2007309351 @default.
- W2268682485 hasRelatedWork W2007339989 @default.
- W2268682485 hasRelatedWork W2009274492 @default.
- W2268682485 hasRelatedWork W2021968271 @default.
- W2268682485 hasRelatedWork W2032265718 @default.
- W2268682485 hasRelatedWork W2035964163 @default.
- W2268682485 hasRelatedWork W2045208059 @default.
- W2268682485 hasRelatedWork W2052660114 @default.
- W2268682485 hasRelatedWork W2060254675 @default.
- W2268682485 hasRelatedWork W2107094329 @default.
- W2268682485 hasRelatedWork W2140327169 @default.
- W2268682485 hasRelatedWork W2341537697 @default.
- W2268682485 hasRelatedWork W2528720459 @default.
- W2268682485 hasRelatedWork W2741705539 @default.
- W2268682485 hasRelatedWork W2742036340 @default.
- W2268682485 hasRelatedWork W2742087921 @default.
- W2268682485 hasRelatedWork W2890968367 @default.
- W2268682485 hasVolume "48" @default.
- W2268682485 isParatext "false" @default.
- W2268682485 isRetracted "false" @default.
- W2268682485 magId "2268682485" @default.
- W2268682485 workType "article" @default.