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• W2269049259 abstract "Switchgrass (Panicum virgatum L.) is an important bioenergy crop. A reliable and efficient transformation method is required to assist with molecular breeding of this crop. In this study, we established a simple and efficient Agrobacterium-mediated transformation method for caryopsis-derived Type I callus by optimizing the cocultivation (7 days at 22°C on medium supplemented with 10 g l−1 glucose and 100 µM acetosyringone) and preculture (2 weeks on medium supplemented with 5 g l−1 casamino acids after cell straining) conditions without the need for time-consuming treatments before and after cocultivation. The present transformation method was successfully applied to different genotypes of switchgrass including a recalcitrant lowland cultivar. The transformation efficiencies of callus lines of the lowland cultivars ‘Alamo’ and ‘Kanlow’ were 12.5–59% and 6.3–20%, respectively. An upland cultivar ‘Trailblazer’ formed Type I calli and one of three tested callus lines produced transgenic plants at relatively high efficiency (7.5%). In contrast, Type I callus formation was unsuccessful for two other upland cultivars, ‘Blackwell’ and ‘Cave-in-Rock.’ This simple and efficient transformation method is suitable for routine and large-scale experiments due to its ready availability of caryopses, prevalence of Type I callus formation, and longevity in the regeneration ability of the callus." @default.
• W2269049259 created "2016-06-24" @default.
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• W2269049259 date "2016-01-01" @default.
• W2269049259 modified "2023-09-30" @default.
• W2269049259 title "An efficient <i>Agrobacterium</i>-mediated transformation method for switchgrass genotypes using Type I callus" @default.
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• W2269049259 doi "https://doi.org/10.5511/plantbiotechnology.15.1127b" @default.
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