Matches in SemOpenAlex for { <https://semopenalex.org/work/W2269191513> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2269191513 abstract "A vaccine expressing virus-like particles is an attractive candidate for the development of an effective vaccine for human immunodeficiency virus type 1 (HIV-1). A single vaccine plasmid was constructed to express HIV-1 Gag, Pol, Env, Tat, Rev and Vpu. Safety mutations and deletions were introduced into the VLP DNA to generate a vaccine insert that was non-infectious. The 5i¦and 3i¦ long terminal repeats, integrase, vif, vpr and nef were removed to further enhance the safety of the vaccine insert. Moreover, mutations were introduced into nucleocapsid and reverse transcriptase to severely restrict viral RNA packaging and to abolish RT and RNase H activity. Virus-like particles were efficiently released from primate cells, but particles were not produced in rodent cells. Therefore, purified particles were used as the inoculum to test the immunogenicity of the VLP vaccines in a rodent system. Systemic and mucosal immune responses to HIV-1 were enhanced by intranasal immunization of purified VLPs expressed from the virally-regulated multi-gene DNA vaccine. VLPs were co-immunized with cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODNs) to enhance the immune response to HIV-1 gene products. VLPs elicited specific immunity to HIV-1 antigens in both the systemic and mucosal immune compartments. Anti-Env antibodies were detected in the sera, as well as in the washes from harvested lungs, intestines and vagina from immunized mice. In addition, Env- and Gag-specific IFN-ƒ×-secreting splenocytes were elicited in the mice vaccinated with VLPs. Co-inoculation of CpG ODNs with VLPs significantly enhanced both arms of the immune response. In addition, these particulate immunogens were compared to soluble proteins (Gag and Env). Mice immunized with soluble protein alone or co-vaccinated with CpG ODNs elicited much lower immune responses compared to VLP-vaccinated mice. Specifically, CTLs were induced by vaccination with VLPs, whereas the immune response elicited by soluble proteins (+/- CpG ODNs) was almost exclusively antibody-mediated. Overall, a vaccine expressing virus-like particles is one of the most promising alternatives to replication-competent virus in eliciting high levels of cross-reactive neutralizing antibodies in combination with a robust cell-mediated response against multiple viral antigens to protect an HIV-infected host from life-long infection or disease." @default.
- W2269191513 created "2016-06-24" @default.
- W2269191513 creator A5067150179 @default.
- W2269191513 date "2005-08-11" @default.
- W2269191513 modified "2023-09-27" @default.
- W2269191513 title "Construction, Characterization and Immunogenicity of Human Immunodeficiency Virus-Like Particles" @default.
- W2269191513 hasPublicationYear "2005" @default.
- W2269191513 type Work @default.
- W2269191513 sameAs 2269191513 @default.
- W2269191513 citedByCount "0" @default.
- W2269191513 crossrefType "journal-article" @default.
- W2269191513 hasAuthorship W2269191513A5067150179 @default.
- W2269191513 hasConcept C104317684 @default.
- W2269191513 hasConcept C147483822 @default.
- W2269191513 hasConcept C159047783 @default.
- W2269191513 hasConcept C159654299 @default.
- W2269191513 hasConcept C167508917 @default.
- W2269191513 hasConcept C203014093 @default.
- W2269191513 hasConcept C2522874641 @default.
- W2269191513 hasConcept C2776387124 @default.
- W2269191513 hasConcept C2780801004 @default.
- W2269191513 hasConcept C2780868878 @default.
- W2269191513 hasConcept C40767141 @default.
- W2269191513 hasConcept C55493867 @default.
- W2269191513 hasConcept C86803240 @default.
- W2269191513 hasConcept C8891405 @default.
- W2269191513 hasConceptScore W2269191513C104317684 @default.
- W2269191513 hasConceptScore W2269191513C147483822 @default.
- W2269191513 hasConceptScore W2269191513C159047783 @default.
- W2269191513 hasConceptScore W2269191513C159654299 @default.
- W2269191513 hasConceptScore W2269191513C167508917 @default.
- W2269191513 hasConceptScore W2269191513C203014093 @default.
- W2269191513 hasConceptScore W2269191513C2522874641 @default.
- W2269191513 hasConceptScore W2269191513C2776387124 @default.
- W2269191513 hasConceptScore W2269191513C2780801004 @default.
- W2269191513 hasConceptScore W2269191513C2780868878 @default.
- W2269191513 hasConceptScore W2269191513C40767141 @default.
- W2269191513 hasConceptScore W2269191513C55493867 @default.
- W2269191513 hasConceptScore W2269191513C86803240 @default.
- W2269191513 hasConceptScore W2269191513C8891405 @default.
- W2269191513 hasLocation W22691915131 @default.
- W2269191513 hasOpenAccess W2269191513 @default.
- W2269191513 hasPrimaryLocation W22691915131 @default.
- W2269191513 hasRelatedWork W1619086299 @default.
- W2269191513 hasRelatedWork W1666555118 @default.
- W2269191513 hasRelatedWork W1907536771 @default.
- W2269191513 hasRelatedWork W1969184830 @default.
- W2269191513 hasRelatedWork W1998228319 @default.
- W2269191513 hasRelatedWork W2005580960 @default.
- W2269191513 hasRelatedWork W2014578259 @default.
- W2269191513 hasRelatedWork W2027610059 @default.
- W2269191513 hasRelatedWork W2042437669 @default.
- W2269191513 hasRelatedWork W2061288068 @default.
- W2269191513 hasRelatedWork W2079890438 @default.
- W2269191513 hasRelatedWork W2104887267 @default.
- W2269191513 hasRelatedWork W2109030288 @default.
- W2269191513 hasRelatedWork W2123909340 @default.
- W2269191513 hasRelatedWork W2183818412 @default.
- W2269191513 hasRelatedWork W2608099443 @default.
- W2269191513 hasRelatedWork W2749258030 @default.
- W2269191513 hasRelatedWork W2914156050 @default.
- W2269191513 hasRelatedWork W3161550638 @default.
- W2269191513 hasRelatedWork W3200767913 @default.
- W2269191513 isParatext "false" @default.
- W2269191513 isRetracted "false" @default.
- W2269191513 magId "2269191513" @default.
- W2269191513 workType "article" @default.