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- W2269558583 abstract "A method of DNA amplification fingerprinting (DAF) based on the DNA polymerase chain reaction (PCR) has been developed to identify different varieties of Phalaenopsis. This method used DNA extracted from trace amounts of leaf material. Distinguishable DAF patterns among the varieties with similar genetic background were obtainable if a suitable primer was used. These DAF patterns serve as molecular markers to protect patent rights to new Phalaenopsis varieties from Taiwan Sugar Corporation. Crosses were made between white and red floral varieties of Phalaenopsis equestris and Doritis pulcherrima. F1 and parental plant were screened with 360 primers in PCR to analyze their DAF patterns. Results showed that 8 primers in Phalaenopsis equestris and 7 primers in Doritis pulcherrima produced distinct DNA polymorphism bands in red floral parent and F1 progeny, but not in white floral parent. These molecular markers will be used for selection of progenies with red flowers at young seedling stage." @default.
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- W2269558583 date "1995-01-01" @default.
- W2269558583 modified "2023-09-26" @default.
- W2269558583 title "Application of DNA Amplification Fingerprinting in the Breeding of Phalaenopsis Orchid" @default.
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- W2269558583 doi "https://doi.org/10.1007/978-94-011-0307-7_47" @default.
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