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- W2269921627 abstract "[Objective]This study aimed to clone and express formyltransferase( Wbkc) gene from Brucella abortus in E. coli,purify the expressed protein and analyze its immunogenicity. [Method]A gene encoding 27- 35 ku formyltransferase( Wbkc) was amplified from the genomic DNA of Brucella abortus by PCR.The amplified fragments were digested with BamH I and Sal I,and inserted into pET28a vector. The constructed recombinant plasmid pET 28a-Wbkc was transformed into E. coli BL21 and was induced to express the fusion protein. Subsequently,the protein was purified by histidine-binding resin column chromatography,and the immunogenicity was detected by Western blot assay. The recombinant plasmid was identified by colony PCR,double digestion and sequencing analysis.[Result]Wbkc was successfully cloned and expressed in E. coli. A specific protein band of 29 ku was detected by SDS-PAGE. Western blot showed specific immunoreactivity of the purified fusion protein. [Conclusion]This study provided a solid foundation for further investigating diagnostic proteins for brucellosis and developing Brucella gene-deletion vaccines." @default.
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- W2269921627 date "2014-01-01" @default.
- W2269921627 modified "2023-09-22" @default.
- W2269921627 title "Prokaryotic Expression and Antigenic Analysis of Wbkc Gene from Brucella abortus" @default.
- W2269921627 hasPublicationYear "2014" @default.
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