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- W2270045947 abstract "The Arabidopsis thaliana D-12 fatty acid desaturase gene (FAD2) was overexpressed in Saccharomyces cerevisiae by using the GAL1 promoter. S. cerevisiae harboring the FAD2 gene was capable of forming hexadecadienoyl (16:2) and linoleoyl (18:2) residues in the membrane lipid when cultured in medium containing galactose. Gas-liquid chromatography analysis of total lipids indicated that the transformed S. cerevisiae accumulated these dienoic fatty acyl residues and that they accounted for approximately 50% of the total fatty acyl residues. Phospholipid analysis of this strain indicated that the oleoyl (18:1) residue binding phosphatidylcholine (PC) was mostly converted to the 18:2 residue binding PC, whereas 50% of the palmitoleoyl (16:1) residue binding PC was converted to the 16:2 residue binding PC. A marked effect on the unsaturation of 16:1 and 18:1 was observed whenS. cerevisiaeharboring theFAD2gene was cultured at 8&C. To assess the ethanol tolerance ofS. cerevisiaeproducing polyunsaturated fatty acids, the cell viability of this strain in the presence ofethanolwasexamined.TheresultsindicatedthatS.cerevisiaecellsoverexpressingtheFAD2genehadgreater resistance to 15% (vol/vol) ethanol than did the control cells." @default.
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- W2270045947 date "1996-01-01" @default.
- W2270045947 modified "2023-09-24" @default.
- W2270045947 title "Polyunsaturated Fatty Acid Biosynthesis inSaccharomyces cerevisiae: Expression of Ethanol Tolerance and theFAD2 Gene fromArabidopsis thaliana" @default.
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