Matches in SemOpenAlex for { <https://semopenalex.org/work/W2270249610> ?p ?o ?g. }
- W2270249610 abstract "Abstract In bacteria, selective promoter recognition by RNA polymerase is achieved by its association with σ factors, accessory subunits able to direct RNA polymerase “core enzyme” (E) to different promoter sequences. Using Chromatin Immunoprecipitation-sequencing (ChIP-seq), we searched for promoters bound by the σ S -associated RNA polymerase form (Eσ S ) during transition from exponential to stationary phase. We identified 63 binding sites for Eσ S overlapping known or putative promoters, often located upstream of genes (encoding either ORFs or non-coding RNAs) showing at least some degree of dependence on the σ S -encoding rpoS gene. Eσ S binding did not always correlate with an increase in transcription level, suggesting that, at some σ S -dependent promoters, Eσ S might remain poised in a pre-initiation state upon binding. A large fraction of Eσ S -binding sites corresponded to promoters recognized by RNA polymerase associated with σ 70 or other σ factors, suggesting a considerable overlap in promoter recognition between different forms of RNA polymerase. In particular, Eσ S appears to contribute significantly to transcription of genes encoding proteins involved in LPS biosynthesis and in cell surface composition. Finally, our results highlight a direct role of Eσ S in the regulation of non coding RNAs, such as OmrA/B, RyeA/B and SibC." @default.
- W2270249610 created "2016-06-24" @default.
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- W2270249610 date "2015-05-28" @default.
- W2270249610 modified "2023-10-18" @default.
- W2270249610 title "Characterization of the Escherichia coli σS core regulon by Chromatin Immunoprecipitation-sequencing (ChIP-seq) analysis" @default.
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- W2270249610 doi "https://doi.org/10.1038/srep10469" @default.
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