Matches in SemOpenAlex for { <https://semopenalex.org/work/W227058148> ?p ?o ?g. }
Showing items 1 to 53 of
53
with 100 items per page.
- W227058148 endingPage "112" @default.
- W227058148 startingPage "109" @default.
- W227058148 abstract "Cell cultures can be contaminated with species belonging to the mycoplasma genus. Contaminating mycoplasmas may alter host cellular characteristics, for instance, alterations in growth characteristics, enzyme patterns, cell membrane composition and chromosomal abnormalities. These drawbacks emphasize the need to regularly screen cell cultures for mycoplasmal contamination. Objective: Mycoplasma detection in cell cultures came from several biomedical laboratories. Material and methods: Cells culture, were grown in absence of antibiotic and washed with PBS solution. Monolayer cells were first scraped off and suspended in 2 mL of SP-4 broth. Samples included in the microbiological culture were analyzed by PCR. Results: The microbiological study showed that 62.5% of the cultures was positive for the mycoplasma isolated and 37.5% negative. PCR technique determined the specific fragment (301 bp) for the mycoplasma genus. Conclusion: Because the mycoplasma frequently contaminate cell cultures, continuous detection is suggested, mainly in biomedical laboratories." @default.
- W227058148 created "2016-06-24" @default.
- W227058148 creator A5004429503 @default.
- W227058148 creator A5035047324 @default.
- W227058148 creator A5067800287 @default.
- W227058148 date "2006-01-01" @default.
- W227058148 modified "2023-09-23" @default.
- W227058148 title "Contaminación por micoplasmas en cultivos celulares" @default.
- W227058148 cites W1503431173 @default.
- W227058148 cites W1512582951 @default.
- W227058148 cites W1549381145 @default.
- W227058148 cites W1991291974 @default.
- W227058148 cites W2012727344 @default.
- W227058148 cites W2013892338 @default.
- W227058148 cites W2035677261 @default.
- W227058148 cites W2069695821 @default.
- W227058148 cites W2121244310 @default.
- W227058148 cites W2147552814 @default.
- W227058148 cites W2896954501 @default.
- W227058148 cites W57786343 @default.
- W227058148 hasPublicationYear "2006" @default.
- W227058148 type Work @default.
- W227058148 sameAs 227058148 @default.
- W227058148 citedByCount "0" @default.
- W227058148 crossrefType "journal-article" @default.
- W227058148 hasAuthorship W227058148A5004429503 @default.
- W227058148 hasAuthorship W227058148A5035047324 @default.
- W227058148 hasAuthorship W227058148A5067800287 @default.
- W227058148 hasConcept C153911025 @default.
- W227058148 hasConcept C2778838687 @default.
- W227058148 hasConcept C523546767 @default.
- W227058148 hasConcept C54355233 @default.
- W227058148 hasConcept C81885089 @default.
- W227058148 hasConcept C86803240 @default.
- W227058148 hasConcept C89423630 @default.
- W227058148 hasConceptScore W227058148C153911025 @default.
- W227058148 hasConceptScore W227058148C2778838687 @default.
- W227058148 hasConceptScore W227058148C523546767 @default.
- W227058148 hasConceptScore W227058148C54355233 @default.
- W227058148 hasConceptScore W227058148C81885089 @default.
- W227058148 hasConceptScore W227058148C86803240 @default.
- W227058148 hasConceptScore W227058148C89423630 @default.
- W227058148 hasIssue "3" @default.
- W227058148 hasLocation W2270581481 @default.
- W227058148 hasOpenAccess W227058148 @default.
- W227058148 hasPrimaryLocation W2270581481 @default.
- W227058148 hasVolume "51" @default.
- W227058148 isParatext "false" @default.
- W227058148 isRetracted "false" @default.
- W227058148 magId "227058148" @default.
- W227058148 workType "article" @default.