FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2270750413> ?p ?o ?g. }

• W2270750413 endingPage "965" @default.
• W2270750413 startingPage "953" @default.
• W2270750413 abstract "Key points In the dorsal raphe nucleus, it is known that serotonin release activates metabotropic 5‐HT 1A autoreceptors located on serotonin neurons that leads to an inhibition of firing through the activation of G‐protein‐coupled inwardly rectifying potassium channels. We found that in mouse brain slices evoked serotonin release produced a 5‐HT 1A receptor‐mediated inhibitory postsynaptic current (IPSC) that resulted in only a transient pause in firing. While spillover activation of receptors contributed to evoked IPSCs, serotonin reuptake transporters prevented pooling of serotonin in the extrasynaptic space from activating 5‐HT 1A ‐IPSCs. As a result, the decay of 5‐HT 1A ‐IPSCs was independent of the intensity of stimulation or the probability of transmitter release. These results indicate that evoked serotonin transmission in the dorsal raphe nucleus mediated by metabotropic 5‐HT 1A autoreceptors may occur via point‐to‐point synapses rather than by paracrine mechanisms. Abstract In the dorsal raphe nucleus (DRN), feedback activation by Gα i/o ‐coupled 5‐HT 1A autoreceptors reduces the excitability of serotoninergic neurons, which decreases serotonin release both locally within the DRN and in projection regions. Serotonin transmission within the DRN is thought to occur via transmitter spillover and paracrine activation of extrasynaptic receptors. Here, we tested the volume transmission hypothesis in mouse DRN brain slices by recording 5‐HT 1A receptor‐mediated inhibitory postsynaptic currents (5‐HT 1A ‐IPSCs) generated by the activation of G‐protein‐coupled inwardly rectifying potassium channels (GIRKs). We found that in the DRN of ePET1‐EYFP mice, which selectively express enhanced yellow fluorescent protein in serontonergic neurons, the local release of serotonin generated 5‐HT 1A ‐IPSCs in serotonin neurons that rose and fell within a second. The transient activation of 5‐HT 1A autoreceptors resulted in brief pauses in neuron firing that did not alter the overall firing rate. The duration of 5‐HT 1A ‐IPSCs was primarily shaped by receptor deactivation due to clearance via serotonin reuptake transporters. Slowing diffusion with dextran prolonged the rise and reduced the amplitude the IPSCs and the effects were potentiated when uptake was inhibited. By examining the decay kinetics of IPSCs, we found that while spillover may allow for the activation of extrasynaptic receptors, efficient uptake by serotonin reuptake transporters (SERTs) prevented the pooling of serotonin from prolonging the duration of transmission when multiple inputs were active. Together the results suggest that the activation of 5‐HT 1A receptors in the DRN results from the local release of serotonin rather than the extended diffusion throughout the extracellular space." @default.
• W2270750413 created "2016-06-24" @default.
• W2270750413 creator A5002315696 @default.
• W2270750413 creator A5010686469 @default.
• W2270750413 date "2015-12-30" @default.
• W2270750413 modified "2023-10-18" @default.
• W2270750413 title "Mechanisms of 5‐HT_1A receptor‐mediated transmission in dorsal raphe serotonin neurons" @default.
• W2270750413 cites W1516995431 @default.
• W2270750413 cites W1519977525 @default.
• W2270750413 cites W1524233596 @default.
• W2270750413 cites W1552978522 @default.
• W2270750413 cites W1591523569 @default.
• W2270750413 cites W1828538530 @default.
• W2270750413 cites W1903694052 @default.
• W2270750413 cites W1929691281 @default.
• W2270750413 cites W1964433270 @default.
• W2270750413 cites W1968354098 @default.
• W2270750413 cites W1974703596 @default.
• W2270750413 cites W1987083929 @default.
• W2270750413 cites W1988606802 @default.
• W2270750413 cites W1993391624 @default.
• W2270750413 cites W1993861337 @default.
• W2270750413 cites W1994403598 @default.
• W2270750413 cites W1994548103 @default.
• W2270750413 cites W2001003931 @default.
• W2270750413 cites W2001759117 @default.
• W2270750413 cites W2002173877 @default.
• W2270750413 cites W2004000308 @default.
• W2270750413 cites W2005115756 @default.
• W2270750413 cites W2006431157 @default.
• W2270750413 cites W2007077740 @default.
• W2270750413 cites W2013507314 @default.
• W2270750413 cites W2018419775 @default.
• W2270750413 cites W2018557010 @default.
• W2270750413 cites W2020484733 @default.
• W2270750413 cites W2023459285 @default.
• W2270750413 cites W2024905528 @default.
• W2270750413 cites W2027671925 @default.
• W2270750413 cites W2028727536 @default.
• W2270750413 cites W2032141996 @default.
• W2270750413 cites W2033308266 @default.
• W2270750413 cites W2039719761 @default.
• W2270750413 cites W2040763671 @default.
• W2270750413 cites W2041763391 @default.
• W2270750413 cites W2047277297 @default.
• W2270750413 cites W2051541361 @default.
• W2270750413 cites W2065967056 @default.
• W2270750413 cites W2066441222 @default.
• W2270750413 cites W2068401230 @default.
• W2270750413 cites W2069284379 @default.
• W2270750413 cites W2069622944 @default.
• W2270750413 cites W2076883859 @default.
• W2270750413 cites W2081117049 @default.
• W2270750413 cites W2083555839 @default.
• W2270750413 cites W2085215795 @default.
• W2270750413 cites W2085325217 @default.
• W2270750413 cites W2088872749 @default.
• W2270750413 cites W2096681544 @default.
• W2270750413 cites W2103517742 @default.
• W2270750413 cites W2104890744 @default.
• W2270750413 cites W2118127537 @default.
• W2270750413 cites W2127567317 @default.
• W2270750413 cites W2128725941 @default.
• W2270750413 cites W2135662935 @default.
• W2270750413 cites W2138538218 @default.
• W2270750413 cites W2143541037 @default.
• W2270750413 cites W2149687002 @default.
• W2270750413 cites W2156106697 @default.
• W2270750413 cites W2158770881 @default.
• W2270750413 cites W2159612818 @default.
• W2270750413 cites W2167243746 @default.
• W2270750413 cites W2169202485 @default.
• W2270750413 cites W2172245690 @default.
• W2270750413 cites W2305234959 @default.
• W2270750413 cites W2323177083 @default.
• W2270750413 cites W2328241777 @default.
• W2270750413 cites W2419532483 @default.
• W2270750413 cites W86237330 @default.
• W2270750413 doi "https://doi.org/10.1113/jp271716" @default.
• W2270750413 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4753271" @default.
• W2270750413 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26634643" @default.
• W2270750413 hasPublicationYear "2015" @default.
• W2270750413 type Work @default.
• W2270750413 sameAs 2270750413 @default.
• W2270750413 citedByCount "37" @default.
• W2270750413 countsByYear W22707504132016 @default.
• W2270750413 countsByYear W22707504132017 @default.
• W2270750413 countsByYear W22707504132018 @default.
• W2270750413 countsByYear W22707504132019 @default.
• W2270750413 countsByYear W22707504132020 @default.
• W2270750413 countsByYear W22707504132021 @default.
• W2270750413 countsByYear W22707504132022 @default.
• W2270750413 countsByYear W22707504132023 @default.
• W2270750413 crossrefType "journal-article" @default.
• W2270750413 hasAuthorship W2270750413A5002315696 @default.
• W2270750413 hasAuthorship W2270750413A5010686469 @default.
• W2270750413 hasBestOaLocation W22707504131 @default.
• W2270750413 hasConcept C169760540 @default.

• next