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• W2271311287 abstract "The high error rate of next generation sequencing (NGS) restricts some of its applications, such as monitoring virus mutations and detecting rare mutations in tumors. There are two commonly employed sequencing library preparation strategies to improve sequencing accuracy by correcting sequencing errors: read-pairing method and tag-clustering method (i.e. primer ID or UID). Here, we constructed a homogeneous library from a single clone, and compared the variant calling accuracy of these error-correction methods. We comprehensively described the strengths and pitfalls of these methods. We found that both read-pairing and tag-clustering methods significantly decreased sequencing error rate. While the read-pairing method was more effective than the tag-clustering method at correcting insertion and deletion errors, it was not as effective as the tag-clustering method at correcting substitution errors. In addition, we observed that when the read quality was poor, the tag-clustering method led to huge coverage loss. We also tested the effect of applying quality score filtering to the error-correction methods and demonstrated that quality score filtering was able to impose a minor, yet statistically significant improvement to the error-correction methods tested in this study. Our study provides a benchmark for researchers to select suitable error-correction methods based on the goal of the experiment by balancing the trade-off between sequencing cost (i.e. sequencing coverage requirement) and detection sensitivity." @default.
• W2271311287 created "2016-06-24" @default.
• W2271311287 creator A5020079595 @default.
• W2271311287 creator A5029398617 @default.
• W2271311287 creator A5037737527 @default.
• W2271311287 date "2016-02-12" @default.
• W2271311287 modified "2023-10-09" @default.
• W2271311287 title "A benchmark study on error-correction by read-pairing and tag-clustering in amplicon-based deep sequencing" @default.
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• W2271311287 doi "https://doi.org/10.1186/s12864-016-2388-9" @default.
• W2271311287 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4751728" @default.
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• W2271311287 hasPublicationYear "2016" @default.
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