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- W2271943530 abstract "Staphylococus aureus and coagulase-negative staphylococci (CNS) are recognized as causing nosocomial and community-acquired infection in every region of the world. We study biofilm production in 43 isolates of methicillin-resistant Staphylococcus aureus (MRSA) and 21 isolates methicillin-resistant Staphylococcus epidermidis (MRSE) and the antibiotics susceptibilities of these strains that were obtained from infected patients at Antonio Pedro University Hospital (HUAP) located in Niteroi city, Rio de Janeiro. In addition, Pulsed-field gel electrophoresis (PFGE) of SmaI macrorestriction fragments of genomic DNA as well as staphylococcal cassette chromosome mec (SCCmec) typing for mecA-carrying isolates were used to study the distribution of clonal types among 43 MRSA recovered in HUAP between 2003 and 2006. Our results demonstrated that the majority strains of MRSA (83.7%) and MRSE (52,4%) produced biofilm and high resistance of MRSA and MRSE to erytromicin, clindamicin and ciprofloxacin were observed. The two major clones types (A and B) were identified by PFGE, with both them comprising the majority of strains (32,6% each). According to SCCmec typing, SCCmec type IV were the most prevalent type, showing 51,2% . But the most interesting in our study is a changed observed in the genetic background of MRSA isolates from HUAP, because of Brazilian clone, which was the major clone in this hospital, was replaced by the USA-400, one type of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA)." @default.
- W2271943530 created "2016-06-24" @default.
- W2271943530 creator A5036429348 @default.
- W2271943530 date "2008-01-01" @default.
- W2271943530 modified "2023-09-23" @default.
- W2271943530 title "Caracterização de cepas de staphylococcus resistentes à meticilina quanto à produção de biofilme, resistência a antimicrobianos e realização do perfil e da tipificação clonal" @default.
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