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• W2272202342 abstract "ix Introduction 1 HIV and AIDS 1 MAIDS model system 3 Role of CD8 T-cells 5 Interleukin -2 8 Interleukin-2 Receptor 10 Purpose and Expectations 10 Materials and Methods 13 Virus and Mice 13 Leukocyte Isolation from Spleens 13 Culturing Cells for Cytokine Assays 14 CD8 T-cell Isolation 15 Freezing and Thawing Cells 17 Antibody Concentrations for Flow Cytometry 18 Antibody Staining 19 Results 22 CD8 Expression for Naive and 1-week Infected Mice 22 Antibody Optimizations for APC-labeled anti-IL-2Rα, Control Antibodies, and CellTracker Green 28 CD8 T-cell Isolation and IL-2R Experiments 29 Flow cytometry Using Antibodies to CD8 and CD25 42 CD25 Expression on Unseparated Leukocytes from 2-week Infected Mice 47 Discussion 54 Problems Encountered 54 CD8 Expression 56 High Affinity IL-2R Expression on CD8 T-cells 57 CD25 Expression on 2-week Infected Spleen Leukocytes 60 Future Work and Conclusions 63 References 66 Appendix LIST OF FIGURES Page Figure 1. Spleen leukocytes from naive BALB/c and C57BL/6 mice stained with the CD8 antibody or corresponding control antibody 25 Figure 2. Spleen leukocytes from 1 week infected BALB/c and C57BL/6 mice stained with the CD8 antibody or corresponding control antibody. ..26 Figure 3. Percent of live spleen cells labeled with the APC antibody for naive and 1-week infected BALB/c and C57BL/6 mice. 27 Figure 4. Isolated CD8 cells from the spleens of a naive BALB/c mouse and a naive C57BL/6 mouse stained with the CD25 antibody or corresponding control antibody. 33 Figure 5. Isolated CD8 cells from the spleens of a 1-week infected BALB/c mouse and a 1-week infected C57BL/6 mouse stained with the CD25 antibody or corresponding control antibody. 34 Figure 6. Isolated CD8 cells from the spleens of a 2-week infected C57BL/6 mouse stained with the CD25 antibody or corresponding control antibody 35 Figure 7. Isolated CD8 cells from the spleens of a 4-week infected BALB/c mouse and a 4-week infected C57BL/6 mouse stained with the CD25 antibody or corresponding control antibody 36 Figure 8. Percent of CD8 cells expressing CD25 for naive, 1-week, 2-week and 4-week infected BALB/c and C57BL/6 mice 37 Figure 9. Leukocytes from the spleen of a naive BALB/c mouse and a naive C57BL/6 mouse stained with the CD25 antibody or corresponding control antibody. 40 Figure 10. Leukocytes from the spleens of 2-week infected BALB/c and C57BL/6 mice stained with the CD25 antibody or corresponding control antibody 41" @default.
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• W2272202342 date "2011-02-16" @default.
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• W2272202342 title "CD8+ T-cell Response Potential, as Determined by Expression of the High Affinity Interleukin-2 Receptor, in Murine AIDS" @default.
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