Matches in SemOpenAlex for { <https://semopenalex.org/work/W2272836728> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2272836728 endingPage "580" @default.
- W2272836728 startingPage "580" @default.
- W2272836728 abstract "The tuberculin purified protein derivative (PPD) is a widely used diagnostic antigen for tuberculosis. It consists of more than 100 denatured proteins in a culture filtrate of a heated culture of Mycobacterium tuberculosis. In two-dimensional electrophoretic analysis of PPDs from M. tuberculosis and M. bovis BCG, most proteins were diffusely separated and could not be seen as spots because of denaturation, whereas a few proteins showed relatively clear spots, indicating heat resistance. Two such proteins corresponded to ribosomal proteins L7 and L12. The mixture of these proteins L7/L2 induced a strong delayed-type hypersensitivity reaction. Another protein showing a clear spot was a GroES analogue, but this did not induce delayed-type hypersensitivity. There were a few other unidentified proteins. It is well known that L7 and L12 are encoded by the same gene and that they differ from each other only by an acetylic post-translational modification that occurs at the N-terminus of L12 converting it to L7 in Escherichia coli. L12, but not L7, was found in two-dimensional electrophoresis of BCG ribosomes, although we found two proteins corresponding to L7 and L12 in PPDs and a native culture filtrate of BCG. We compared the delayed-type hypersensitivity reaction elicited by L7/L12 derived from a culture filtrate of BCG and L12 derived from BCG ribosomes. L7/L12 from the culture filtrate could induce delayed-type hypersensitivity, but L12 from ribosomes could not, indicating that L7 was attributable to the induction of delayed-type hypersensitivity. The activity of L7/L12 was heat resistant. Neither glycosylation nor phosphorylation of L7/L12 from a culture filtrate could be detected. The acetylation at N-terminal of L12 was essential for the delayed-type hypersensitivity activity." @default.
- W2272836728 created "2016-06-24" @default.
- W2272836728 creator A5006647628 @default.
- W2272836728 date "1999-12-01" @default.
- W2272836728 modified "2023-10-14" @default.
- W2272836728 title "Basic Immunology Ribosomal Protein L7 Included in Tuberculin Purified Protein Derivative (PPD) is a Major Heat-Resistant Protein Inducing Strong Delayed-Type Hypersensitivity" @default.
- W2272836728 doi "https://doi.org/10.1046/j.1365-3083.1999.00630.x" @default.
- W2272836728 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10607306" @default.
- W2272836728 hasPublicationYear "1999" @default.
- W2272836728 type Work @default.
- W2272836728 sameAs 2272836728 @default.
- W2272836728 citedByCount "19" @default.
- W2272836728 countsByYear W22728367282012 @default.
- W2272836728 countsByYear W22728367282013 @default.
- W2272836728 countsByYear W22728367282014 @default.
- W2272836728 countsByYear W22728367282015 @default.
- W2272836728 crossrefType "journal-article" @default.
- W2272836728 hasAuthorship W2272836728A5006647628 @default.
- W2272836728 hasConcept C104317684 @default.
- W2272836728 hasConcept C142724271 @default.
- W2272836728 hasConcept C147483822 @default.
- W2272836728 hasConcept C153911025 @default.
- W2272836728 hasConcept C185592680 @default.
- W2272836728 hasConcept C203014093 @default.
- W2272836728 hasConcept C2776247216 @default.
- W2272836728 hasConcept C2777975735 @default.
- W2272836728 hasConcept C2778497218 @default.
- W2272836728 hasConcept C2781069245 @default.
- W2272836728 hasConcept C2910580931 @default.
- W2272836728 hasConcept C3017924640 @default.
- W2272836728 hasConcept C38062823 @default.
- W2272836728 hasConcept C55493867 @default.
- W2272836728 hasConcept C67705224 @default.
- W2272836728 hasConcept C71924100 @default.
- W2272836728 hasConcept C86803240 @default.
- W2272836728 hasConcept C88478588 @default.
- W2272836728 hasConcept C89423630 @default.
- W2272836728 hasConceptScore W2272836728C104317684 @default.
- W2272836728 hasConceptScore W2272836728C142724271 @default.
- W2272836728 hasConceptScore W2272836728C147483822 @default.
- W2272836728 hasConceptScore W2272836728C153911025 @default.
- W2272836728 hasConceptScore W2272836728C185592680 @default.
- W2272836728 hasConceptScore W2272836728C203014093 @default.
- W2272836728 hasConceptScore W2272836728C2776247216 @default.
- W2272836728 hasConceptScore W2272836728C2777975735 @default.
- W2272836728 hasConceptScore W2272836728C2778497218 @default.
- W2272836728 hasConceptScore W2272836728C2781069245 @default.
- W2272836728 hasConceptScore W2272836728C2910580931 @default.
- W2272836728 hasConceptScore W2272836728C3017924640 @default.
- W2272836728 hasConceptScore W2272836728C38062823 @default.
- W2272836728 hasConceptScore W2272836728C55493867 @default.
- W2272836728 hasConceptScore W2272836728C67705224 @default.
- W2272836728 hasConceptScore W2272836728C71924100 @default.
- W2272836728 hasConceptScore W2272836728C86803240 @default.
- W2272836728 hasConceptScore W2272836728C88478588 @default.
- W2272836728 hasConceptScore W2272836728C89423630 @default.
- W2272836728 hasIssue "6" @default.
- W2272836728 hasLocation W22728367281 @default.
- W2272836728 hasLocation W22728367282 @default.
- W2272836728 hasOpenAccess W2272836728 @default.
- W2272836728 hasPrimaryLocation W22728367281 @default.
- W2272836728 hasRelatedWork W1981881107 @default.
- W2272836728 hasRelatedWork W2066898978 @default.
- W2272836728 hasRelatedWork W2141427212 @default.
- W2272836728 hasRelatedWork W2160333531 @default.
- W2272836728 hasRelatedWork W2347255347 @default.
- W2272836728 hasRelatedWork W2410707153 @default.
- W2272836728 hasRelatedWork W2412709800 @default.
- W2272836728 hasRelatedWork W2561250975 @default.
- W2272836728 hasRelatedWork W2781994832 @default.
- W2272836728 hasRelatedWork W344982219 @default.
- W2272836728 hasVolume "50" @default.
- W2272836728 isParatext "false" @default.
- W2272836728 isRetracted "false" @default.
- W2272836728 magId "2272836728" @default.
- W2272836728 workType "article" @default.