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• W2273010484 abstract "To investigate the effects of Ca2 + activated potassium channel KCa3.1 and voltage-gated potassium channel Kv1.3 of B lymphocyte on inflammatory monocytes chemotaxis and the potential mechanisms. Thanswell test was used to detect the inflammatory monocyte (Ly-6Chi) chemotaxis caused by the B lymphocyte. Enzyme-linked immunosorbent assay (ELISA) was applied to detecting the C-C motif ligand 7 (CCL7) in cultured media. Cell counting kit-8 (CCK) was used to detect the proliferation of B lymphocytes after activation and blockage of both KCa3.1 and Kv1.3 channels. Western blot was used to detect the expression of phosphorylated extracellular signal-regulated kinase (P-ERK) of the B lymphocytes. When activated, B lymphocytes significantly proliferated. After application of KCa3.1 channel-specific inhibitor TRAM-34 and potent Kv1.3 channel inhibitor ShK, both B lymphocytes proliferation and Ly-6Chi monocyte chemotaxis were significantly inhibited. The expression of chemotaxis related factor CCL7 decreased remarkably. The opening of KCa3.1 and Kv1.3 channels promote B lymphocyte activation, proliferation and Ly-6Chi monocyte chemotaxis. The increase of CCL7 secretion by B lymphocyte may explain the pro migration effects." @default.
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• W2273010484 date "2016-02-01" @default.
• W2273010484 modified "2023-10-09" @default.
• W2273010484 title "Blockage of KCa3.1 and Kv1.3 channels of the B lymphocyte decreases the inflammatory monocyte chemotaxis" @default.
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• W2273010484 doi "https://doi.org/10.1016/j.intimp.2015.12.032" @default.
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