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- W2273313888 abstract "[Objective] The aim was to establish a rapid method by HPLC for determination of caffeine concentration in tea.[Method] The conditions were: chromatographic column Zorbax-C8(4.6 mm×250.0 mm,5.0 μm),mobile phase consisting of a mixture of citric acid solution(0.04 mol/L)∶N,N-dimethyl formamide∶tetrahydrofuran=90∶16∶4,flow rate 1.00 ml/min,column temperature 60 ℃,detection wavelength 280 nm,the sample dose 10 μl.[Result] When the concentration of caffeine was 20-100 μg/ ml,the perfect linearity between concentration and peak area ratio was obtained(r=0.999 9).The recovery rate was 98.9%-102.3%,the relative standard deviation was 0.23%.[Conclusion] The method is simple,rapid and accurate,and can be used for determination of caffeine concentration in tea and tea drinks." @default.
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- W2273313888 date "2010-01-01" @default.
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- W2273313888 title "Determination of Caffeine in Tea by HPLC" @default.
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