Matches in SemOpenAlex for { <https://semopenalex.org/work/W2273434350> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2273434350 endingPage "15" @default.
- W2273434350 startingPage "13" @default.
- W2273434350 abstract "Objective To investigate integration and expression of adeno-associated virus (AAV) vectors in neuronal PC12 cells,the result of which can be applied in further gene therapy of diseases of the central nervous system. Methods Human neurotrophin-3(hNT3)genes were inserted into AAV vectors. Then the recombinat AAV plasmids were encapsidated as recombinant virions. PC12 cells were transfected with the virions and the positive cells were selected by G418. The transfection positive (hNT3 modified)PC12 cells were cultured for several generations and the cellular genomic DNA and total RNA were extracted. We investigated the integration locus of AAV vectors by Southern blot and transcript situation of foreign genes by dot blot. Results The hybridization tests showed that AAV introduced foreign genes were stably integrated, but at random locus, and robustly transcribed in hNT3 modified PC12cells. Conclusion AAV vectors can serve as high efficiency vectors of target genes in neuronal PC12 cells." @default.
- W2273434350 created "2016-06-24" @default.
- W2273434350 creator A5034827133 @default.
- W2273434350 creator A5066455265 @default.
- W2273434350 date "2001-01-01" @default.
- W2273434350 modified "2023-09-22" @default.
- W2273434350 title "ADENO-ASSOCIATED VIRUS INTRODUCED INTEGRATION AND EXPRESSION OF FOREIGN GENES IN PC12 CELLS" @default.
- W2273434350 hasPublicationYear "2001" @default.
- W2273434350 type Work @default.
- W2273434350 sameAs 2273434350 @default.
- W2273434350 citedByCount "0" @default.
- W2273434350 crossrefType "journal-article" @default.
- W2273434350 hasAuthorship W2273434350A5034827133 @default.
- W2273434350 hasAuthorship W2273434350A5066455265 @default.
- W2273434350 hasConcept C104317684 @default.
- W2273434350 hasConcept C111599444 @default.
- W2273434350 hasConcept C133307689 @default.
- W2273434350 hasConcept C153911025 @default.
- W2273434350 hasConcept C159047783 @default.
- W2273434350 hasConcept C22744801 @default.
- W2273434350 hasConcept C2522874641 @default.
- W2273434350 hasConcept C2778107364 @default.
- W2273434350 hasConcept C40767141 @default.
- W2273434350 hasConcept C54009773 @default.
- W2273434350 hasConcept C54355233 @default.
- W2273434350 hasConcept C84597430 @default.
- W2273434350 hasConcept C86803240 @default.
- W2273434350 hasConcept C92087593 @default.
- W2273434350 hasConceptScore W2273434350C104317684 @default.
- W2273434350 hasConceptScore W2273434350C111599444 @default.
- W2273434350 hasConceptScore W2273434350C133307689 @default.
- W2273434350 hasConceptScore W2273434350C153911025 @default.
- W2273434350 hasConceptScore W2273434350C159047783 @default.
- W2273434350 hasConceptScore W2273434350C22744801 @default.
- W2273434350 hasConceptScore W2273434350C2522874641 @default.
- W2273434350 hasConceptScore W2273434350C2778107364 @default.
- W2273434350 hasConceptScore W2273434350C40767141 @default.
- W2273434350 hasConceptScore W2273434350C54009773 @default.
- W2273434350 hasConceptScore W2273434350C54355233 @default.
- W2273434350 hasConceptScore W2273434350C84597430 @default.
- W2273434350 hasConceptScore W2273434350C86803240 @default.
- W2273434350 hasConceptScore W2273434350C92087593 @default.
- W2273434350 hasIssue "1" @default.
- W2273434350 hasLocation W22734343501 @default.
- W2273434350 hasOpenAccess W2273434350 @default.
- W2273434350 hasPrimaryLocation W22734343501 @default.
- W2273434350 hasRelatedWork W1617966098 @default.
- W2273434350 hasRelatedWork W1770246221 @default.
- W2273434350 hasRelatedWork W1806895515 @default.
- W2273434350 hasRelatedWork W2015057928 @default.
- W2273434350 hasRelatedWork W2086367339 @default.
- W2273434350 hasRelatedWork W2091924039 @default.
- W2273434350 hasRelatedWork W2096466927 @default.
- W2273434350 hasRelatedWork W2103202783 @default.
- W2273434350 hasRelatedWork W2105679399 @default.
- W2273434350 hasRelatedWork W2148300469 @default.
- W2273434350 hasRelatedWork W2245562270 @default.
- W2273434350 hasRelatedWork W2321725396 @default.
- W2273434350 hasRelatedWork W2351625714 @default.
- W2273434350 hasRelatedWork W2364905448 @default.
- W2273434350 hasRelatedWork W2380102142 @default.
- W2273434350 hasRelatedWork W2388921399 @default.
- W2273434350 hasRelatedWork W2393261645 @default.
- W2273434350 hasRelatedWork W2408358546 @default.
- W2273434350 hasRelatedWork W2413675992 @default.
- W2273434350 hasRelatedWork W2420122227 @default.
- W2273434350 isParatext "false" @default.
- W2273434350 isRetracted "false" @default.
- W2273434350 magId "2273434350" @default.
- W2273434350 workType "article" @default.