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- W2273640888 abstract "Este trabajo se realizo con el objetivo de analizar la diversidad genetica del complejo de especies Ralstonia solanacearum en tres regiones de Colombia donde se siembran cultivos hospedantes de gran importancia economica para el pais. Se colectaron 69 aislamientos procedentes de suelo y diferentes hospedantes. Para determinar la diversidad genetica entre los aislados se utilizaron los marcadores moleculares del tipo microsatelite amplificados al azar (RAMs). Los datos se analizaron utilizando el coeficiente de Nei-Li, el analisis de correspondencia multiple (ACM) y el indice de Shannon. Los valores del coeficiente de Nei-Li, a un nivel de similaridad de 0,76%, permitieron agrupar los aislamientos en tres divisiones. El ACM arrojo un valor de similitud del 67% al interior de 10 grupos y del 62% entre los grupos. El Coeficiente de diferenciacion genetica (Gst) mostro un valor de 0,13 e indico que existe mayor diversidad genetica de los aislados dentro de cada grupo que cuando se comparan los diferentes grupos entre si. El valor del indice de Shannon corresponde con los otros indices y muestra que los aislados en cada grupo presentan una diversidad mayor que la que existe entre los grupos. La interpretacion del valor de Gst interpoblacional (0,0514) sugiere una diferenciacion genetica moderada de la poblacion. Los resultados indican o senalan alto flujo de genes entre las poblaciones. Palabras clave: Ralstonia solanacearum , diversidad genetica, RAMs. Genetic diversity of Ralstonia solanacearum isolates from three Colombian regions Abstract: The present work was performed to analyze the genetic diversity of the R . solanacearum species complex in three Colombian regions where economically important host crops are grown. Sixty nine isolates were collected from different hosts and soils. The genetic diversity was determined using Random Amplified Microsatellites (RAMs) as molecular markers . The data were analyzed by the Nei and Li coefficient, Multiple Correspondence Analysis (MCA), and the Shannon index. The Nei and LI coefficient values found at a similarity level of 0.76% allowed grouping the isolates into three major divisions.. The MCA showed a similarity coefficient of 67% within 10 groups and 62% between groups. The Genetic differentiation coefficient (Gst) showed a value of 0.13 indicating that the genetic diversity was higher within each group of isolates than when the different groups were compared one another. The Shannon index value corresponded with the other calculated indices, indicating that the isolates within each group showed a higher genetic diversity than that observed between the different groups. The Inter-population Gst value (0.0514) suggested a moderate genetic differentiation in the population. The results suggested a high gene flow between populations. Key words: Ralstonia solanacearum, genetic diversity, RAMs." @default.
- W2273640888 created "2016-06-24" @default.
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- W2273640888 date "2015-12-16" @default.
- W2273640888 modified "2023-09-24" @default.
- W2273640888 title "Diversidad genética de aislados de Ralstonia solanacearum procedentes de tres regiones de Colombia" @default.
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