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• W2273646165 endingPage "12" @default.
• W2273646165 startingPage "5" @default.
• W2273646165 abstract "The immunogenic Brucella abortus ribosomal protein L7/L12 and Lipopolysaccharide (LPS) are promising candidate antigens for the development of subunit vaccines against brucellosis.This study was aimed to evaluate the protection of combination of recombinant HSA-L7/L12 fusion protein with LPS in Balb/c mouse.The recombinant HSA-L7/L12 fusion protein in Saccharomyces cerevisiae was expressed and purified by affinity chromatography column. LPS was extracted by n-butanol, purified by ultracentrifugation. BALB/c mouses were immunized in 9 groups with PBS, HSA, tHSA-L7/L12, L7/L12, LPS, LPS+ HSA, LPS+ tHSA-L7/L12, LPS+ L7/L12, B. abortus S19. ELISA, LTT tests and challenging two weeks after last injection were carried out. Bacterial count of spleen of immunized BALB/c mouse was done four weeks after challenging with virulent strain B. abortus 544.In ELISA test the specific antibodies of tHSA-L7/L12 exhibited a dominance of immunoglobulin IgG1 over IgG2a. LPS-HSA and tHSA-L7/L12 + LPS produced a significantly higher antibody titer than LPS alone and L7/L12+LPS (P < 0.05). The predominant IgG subtype for LPS and L7/L12+LPS were IgG3. However, tHSA-L7/L12+ LPS and LPS+ HAS elicited predominantly IgG1 and IgG3 subtypes. In addition, the tHSA-L7/L12 fusion protein and L7/L12 elicited a strong T-cell proliferative response upon restimulation in vitro with recombinant tHSA-L7/L12 and L7/L12, suggesting the induction of a cellular immunity response in vivo. However, there was no significant difference proliferative response in L7/L12 and tHSA-L7/L12 fusion protein (P > 0.05). The combination of tHSA-L7/L12 fusion protein with LPS and B. abortus S19 induce higher level of protection against challenge with the virulent strain B. abortus 544 in BALB/c mice than other groups (P = 0.005).The combination of tHSA-L7/L12 fusion protein with LPS had higher protective ability than LPS and fusion protein distinctly." @default.
• W2273646165 created "2016-06-24" @default.
• W2273646165 creator A5018621305 @default.
• W2273646165 creator A5021813860 @default.
• W2273646165 creator A5052795656 @default.
• W2273646165 creator A5053713032 @default.
• W2273646165 creator A5063686548 @default.
• W2273646165 creator A5071218933 @default.
• W2273646165 date "2010-11-09" @default.
• W2273646165 modified "2023-09-22" @default.
• W2273646165 title "Protection of BALB/C mice against Brucella abortus 544 challenge by vaccination with combination of recombinant human serum albumin-l7/l12 (Brucella abortus ribosomal protein) and lipopolysaccharide." @default.
• W2273646165 cites W112000276 @default.
• W2273646165 cites W12119413 @default.
• W2273646165 cites W1224550439 @default.
• W2273646165 cites W1484280936 @default.
• W2273646165 cites W1485975527 @default.
• W2273646165 cites W1493216 @default.
• W2273646165 cites W1536478289 @default.
• W2273646165 cites W15788867 @default.
• W2273646165 cites W1597678601 @default.
• W2273646165 cites W1602273859 @default.
• W2273646165 cites W1627654352 @default.
• W2273646165 cites W1759442301 @default.
• W2273646165 cites W1797925867 @default.
• W2273646165 cites W1807507789 @default.
• W2273646165 cites W1817642081 @default.
• W2273646165 cites W1852199403 @default.
• W2273646165 cites W1883359496 @default.
• W2273646165 cites W1904767765 @default.
• W2273646165 cites W1907925992 @default.
• W2273646165 cites W1911873988 @default.
• W2273646165 cites W1919924159 @default.
• W2273646165 cites W1932838911 @default.
• W2273646165 cites W1942668068 @default.
• W2273646165 cites W1965302510 @default.
• W2273646165 cites W1966017522 @default.
• W2273646165 cites W1966528088 @default.
• W2273646165 cites W1968218669 @default.
• W2273646165 cites W1971094103 @default.
• W2273646165 cites W1971891090 @default.
• W2273646165 cites W1976464671 @default.
• W2273646165 cites W1977775509 @default.
• W2273646165 cites W1979452014 @default.
• W2273646165 cites W1982402249 @default.
• W2273646165 cites W1982834644 @default.
• W2273646165 cites W1984344504 @default.
• W2273646165 cites W1985782125 @default.
• W2273646165 cites W1987909946 @default.
• W2273646165 cites W1988575792 @default.
• W2273646165 cites W1989994128 @default.
• W2273646165 cites W1990299806 @default.
• W2273646165 cites W1990700333 @default.
• W2273646165 cites W1990902903 @default.
• W2273646165 cites W1991774103 @default.
• W2273646165 cites W1992102076 @default.
• W2273646165 cites W1997159731 @default.
• W2273646165 cites W1997703220 @default.
• W2273646165 cites W1998097661 @default.
• W2273646165 cites W2000461629 @default.
• W2273646165 cites W2005045747 @default.
• W2273646165 cites W2005563588 @default.
• W2273646165 cites W2008588605 @default.
• W2273646165 cites W2015649802 @default.
• W2273646165 cites W2017409119 @default.
• W2273646165 cites W2017690293 @default.
• W2273646165 cites W2019818051 @default.
• W2273646165 cites W2022227920 @default.
• W2273646165 cites W2022276686 @default.
• W2273646165 cites W2023331375 @default.
• W2273646165 cites W2023676090 @default.
• W2273646165 cites W2025315134 @default.
• W2273646165 cites W2029189223 @default.
• W2273646165 cites W2029383937 @default.
• W2273646165 cites W2030244758 @default.
• W2273646165 cites W2033166984 @default.
• W2273646165 cites W2033379859 @default.
• W2273646165 cites W2035126215 @default.
• W2273646165 cites W2035536738 @default.
• W2273646165 cites W2036568358 @default.
• W2273646165 cites W2037002384 @default.
• W2273646165 cites W2039190435 @default.
• W2273646165 cites W2039738824 @default.
• W2273646165 cites W2042987550 @default.
• W2273646165 cites W2045136590 @default.
• W2273646165 cites W2050293562 @default.
• W2273646165 cites W2051866318 @default.
• W2273646165 cites W2052972684 @default.
• W2273646165 cites W2053111481 @default.
• W2273646165 cites W2053231019 @default.
• W2273646165 cites W2055700729 @default.
• W2273646165 cites W2059469939 @default.
• W2273646165 cites W2060959309 @default.
• W2273646165 cites W2062189636 @default.
• W2273646165 cites W2062203307 @default.
• W2273646165 cites W2062992421 @default.
• W2273646165 cites W2064284773 @default.
• W2273646165 cites W2064896248 @default.
• W2273646165 cites W2071710185 @default.

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