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- W2274005891 abstract "For rapid and mass-propagation of rabbiteye blueberry (”Vaccinium ashei”) plants, a protocol for tissue culture was required. The objectives of this study were 1) to investigate the optimal tissue culture condition for rapid proliferation of rabbiteye blueberry plants, and 2) to establish standard operating procedure of tissue culture for rabbiteye blueberry. ”In vitro” derived leaf explants of 'Blueshower', 'Woodard' and 'Tifblue' rabbiteye blueberry were used as plant materials to identify the optimal medium for tissue culture and ”ex vitro” rooting. The results showed that segments from the middle section of a leaf from regenerated axillary shoots were suitable for culture in the medium containing 2.3 μM TDZ. After incubation in the dark for one month and then in the light for another one month, the number of adventitious buds per explant was 15.89 in 'Blueshower', 2.5 in 'Woodard', and 0.17 in 'Tifblue'. The bud clumps of 'Blueshower' were transferred to medium containing 4 μM zeatin and cultured for 1 month. On average, a bud clump generated 2.25 shoots longer than 1 cm. The regenerated shoots were used for ex vitro rooting test. The shoots were dipped in 2000 ppm IBA and inserted in moist rooting mixture of 3 peat moss and 2 perlite (by volume), 80%-90% shoot cuttings rooted after 40 days." @default.
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- W2274005891 date "2014-03-01" @default.
- W2274005891 modified "2023-09-27" @default.
- W2274005891 title "The Establishment of the Plantlet Production of Rabbiteye Blueberry through Tissue Culture from ＂In Vitro＂ Derived Leaf Explants" @default.
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