Matches in SemOpenAlex for { <https://semopenalex.org/work/W2274365514> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2274365514 endingPage "221" @default.
- W2274365514 startingPage "203" @default.
- W2274365514 abstract "Metacaspases are cysteine peptidases found in trypanosomes but absent in mammals, and despite being distantly related to the mammalian caspases they show significant disparity in their cellular and enzymatic functions. The genome of the parasitic protozoa Trypanosoma brucei (the causative agent of African sleeping sickness) encodes five metacaspases: TbMCA1-TbMCA5. Of these TbMCA2, TbMCA3, and TbMCA5 are active cysteine peptidases expressed in the bloodstream form of the parasite. To investigate the structure-function relationship of the trypanosome metacaspases and the structural basis for their divergence from the caspases, paracaspases, and other Clan CD cysteine peptidases (or vice versa), we purified and characterized TbMCA2 and determined the three-dimensional structure of an inactive mutant using X-ray crystallography. The methods presented in this chapter describe the recombinant expression of active TbMCA2 and inactive TbMCA2(C213A). The protocols produce large amounts of recombinant protein for use in structural, biochemical, and kinetic studies and include detailed information on how to produce diffraction quality crystals of TbMCA2(C213A)." @default.
- W2274365514 created "2016-06-24" @default.
- W2274365514 creator A5024173243 @default.
- W2274365514 creator A5080710621 @default.
- W2274365514 creator A5088125040 @default.
- W2274365514 date "2014-01-01" @default.
- W2274365514 modified "2023-09-24" @default.
- W2274365514 title "Purification, Characterization, and Crystallization of Trypanosoma Metacaspases" @default.
- W2274365514 cites W1969615465 @default.
- W2274365514 cites W1987396009 @default.
- W2274365514 cites W1987546203 @default.
- W2274365514 cites W2007226778 @default.
- W2274365514 cites W2009393104 @default.
- W2274365514 cites W2011905899 @default.
- W2274365514 cites W2056155809 @default.
- W2274365514 cites W2060595290 @default.
- W2274365514 cites W2064804935 @default.
- W2274365514 cites W2064881809 @default.
- W2274365514 cites W2070997089 @default.
- W2274365514 cites W2075635047 @default.
- W2274365514 cites W2077115594 @default.
- W2274365514 cites W2086198136 @default.
- W2274365514 cites W2090286595 @default.
- W2274365514 cites W2099721061 @default.
- W2274365514 cites W2120662385 @default.
- W2274365514 cites W2130479394 @default.
- W2274365514 cites W2166356964 @default.
- W2274365514 cites W4232254803 @default.
- W2274365514 doi "https://doi.org/10.1007/978-1-4939-0357-3_13" @default.
- W2274365514 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24567104" @default.
- W2274365514 hasPublicationYear "2014" @default.
- W2274365514 type Work @default.
- W2274365514 sameAs 2274365514 @default.
- W2274365514 citedByCount "6" @default.
- W2274365514 countsByYear W22743655142015 @default.
- W2274365514 countsByYear W22743655142016 @default.
- W2274365514 countsByYear W22743655142017 @default.
- W2274365514 countsByYear W22743655142019 @default.
- W2274365514 countsByYear W22743655142020 @default.
- W2274365514 countsByYear W22743655142021 @default.
- W2274365514 crossrefType "book-chapter" @default.
- W2274365514 hasAuthorship W2274365514A5024173243 @default.
- W2274365514 hasAuthorship W2274365514A5080710621 @default.
- W2274365514 hasAuthorship W2274365514A5088125040 @default.
- W2274365514 hasConcept C104317684 @default.
- W2274365514 hasConcept C143065580 @default.
- W2274365514 hasConcept C159047783 @default.
- W2274365514 hasConcept C181199279 @default.
- W2274365514 hasConcept C190283241 @default.
- W2274365514 hasConcept C2778566189 @default.
- W2274365514 hasConcept C2779201268 @default.
- W2274365514 hasConcept C2779502636 @default.
- W2274365514 hasConcept C2779549974 @default.
- W2274365514 hasConcept C31573885 @default.
- W2274365514 hasConcept C40767141 @default.
- W2274365514 hasConcept C54355233 @default.
- W2274365514 hasConcept C55493867 @default.
- W2274365514 hasConcept C86803240 @default.
- W2274365514 hasConcept C95444343 @default.
- W2274365514 hasConcept C98424977 @default.
- W2274365514 hasConceptScore W2274365514C104317684 @default.
- W2274365514 hasConceptScore W2274365514C143065580 @default.
- W2274365514 hasConceptScore W2274365514C159047783 @default.
- W2274365514 hasConceptScore W2274365514C181199279 @default.
- W2274365514 hasConceptScore W2274365514C190283241 @default.
- W2274365514 hasConceptScore W2274365514C2778566189 @default.
- W2274365514 hasConceptScore W2274365514C2779201268 @default.
- W2274365514 hasConceptScore W2274365514C2779502636 @default.
- W2274365514 hasConceptScore W2274365514C2779549974 @default.
- W2274365514 hasConceptScore W2274365514C31573885 @default.
- W2274365514 hasConceptScore W2274365514C40767141 @default.
- W2274365514 hasConceptScore W2274365514C54355233 @default.
- W2274365514 hasConceptScore W2274365514C55493867 @default.
- W2274365514 hasConceptScore W2274365514C86803240 @default.
- W2274365514 hasConceptScore W2274365514C95444343 @default.
- W2274365514 hasConceptScore W2274365514C98424977 @default.
- W2274365514 hasLocation W22743655141 @default.
- W2274365514 hasLocation W22743655142 @default.
- W2274365514 hasOpenAccess W2274365514 @default.
- W2274365514 hasPrimaryLocation W22743655141 @default.
- W2274365514 hasRelatedWork W1976131495 @default.
- W2274365514 hasRelatedWork W1981415629 @default.
- W2274365514 hasRelatedWork W1994702061 @default.
- W2274365514 hasRelatedWork W2009846929 @default.
- W2274365514 hasRelatedWork W2052075760 @default.
- W2274365514 hasRelatedWork W2089585546 @default.
- W2274365514 hasRelatedWork W2089851507 @default.
- W2274365514 hasRelatedWork W2149189489 @default.
- W2274365514 hasRelatedWork W2153699989 @default.
- W2274365514 hasRelatedWork W2274365514 @default.
- W2274365514 isParatext "false" @default.
- W2274365514 isRetracted "false" @default.
- W2274365514 magId "2274365514" @default.
- W2274365514 workType "book-chapter" @default.