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- W2274739980 abstract "In order to develop a reverse transcription loop-mediated isothermal amplification(RT-LAMP)assay for the rapid detection of hog cholera lapinized virus(HCLV)vaccine strain against classical swine fever,a set of primers,being composed of outer primers and inner primers,was designed for NS5B gene of HCLV using Primer Explorer V4 software.After being amplified at a constant temperature of 65℃ by Bst DNA polymerase,a ladder-like pattern of products was visible on 20g/L agarose gel electrophoresis,and the products could be visualized under the UV light with SYBR GreenⅠ dye.The sensitivity of this assay was evaluated by comparison with the TaqMan real-time RT-PCR described previously.The detection limit of the assay was 5 copies of HCLV per reaction.No cross-reactivity was observed in the samples with other related viruses including different genotypes of wild-type CSFV strains and bovine viral diarrhea virus.The agreement between RT-LAMP and the TaqMan real-time RT-PCR was 100% in detecting 58 HCLV vaccines.The results indicated that the developed RT-LAMP was simple and rapid for the detection of HCLV." @default.
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- W2274739980 date "2010-01-01" @default.
- W2274739980 modified "2023-09-26" @default.
- W2274739980 title "Development of a RT-LAMP method for detection of HCLV vaccine against classical swine fever" @default.
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