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- W2274846886 abstract "Objective The aim of this research was to clone heterotrimeric G protein alpha subunit gene in Setosphaeria turcica and express it in vitro.Method The homologous fragment of Gα gene was isolated by degenerate PCR,the 5'and 3' flanking sequences were cloned using Genome walking,and the full length cDNA was obtained by RT-PCR.Gene structure and putative cis-acting elements were analyzed by bioinformatics methods and then the gene was expressed in vitro by pET expression system.Result The gene named as Stga-2,which encoded a protein of 356 amino acid residues,was 1 318 bp and interrupted by four introns.There wasn't typical TATA-box and CAAT-box in its upstream sequence,while the transcriptional initiator and C+T rich region were observed.Other cis-acting elements,such as Sp1-binding site,CCA motif and CCG repeats,were also found here.At last,Stga-2 was expressed in vitro by pET system.Conclusion Cloning of Stga-2 gene and its promoter region in S.turcica enriched the biological information resource of filamentous fungi,and laid a foundation for the functional analysis about signal transduction pathway in phytopathogenic fungi." @default.
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- W2274846886 date "2010-01-01" @default.
- W2274846886 modified "2023-09-26" @default.
- W2274846886 title "Isolation and Characterization of Stga-2 Gene from Setosphaeria turcica and Its Expression in vitro" @default.
- W2274846886 hasPublicationYear "2010" @default.
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